Weinberger S J, Wright G Z
Faculty of Dentistry, University of Western Ontario, London, Canada.
Pediatr Dent. 1990 Sep-Oct;12(5):312-5.
When saliva is sampled to estimate S. mutans, variables may influence the results. The purpose of the present study was to assess the reliability of the tongue depressor for saliva sampling, and whether the sampling time during the day influences S. mutans counts. The study population consisted of 27 children, between 24 and 66 months of age. Samples of unstimulated saliva were gathered on tongue blades four times during the day for each subject. Paired samples representing both sides of the tongue blades were inoculated onto elevated agar plates containing a selective medium, and anaerobically incubated at 37 degrees C for 48 hr. After examining 76 paired samples of saliva, no significant differences in S. mutans counts were found between sides of the spatula, suggesting that the sampling technique was suitable. Analysis of variance showed significant differences within each subject over the four sampling times (P less than .01). A significant difference was found between subjects' daily averages (P less than .05).
当采集唾液以估计变形链球菌时，一些变量可能会影响结果。本研究的目的是评估用压舌板采集唾液的可靠性，以及一天中的采样时间是否会影响变形链球菌的计数。研究对象为27名年龄在24至66个月之间的儿童。每位受试者在一天中四次用压舌板采集未刺激唾液样本。将代表压舌板两侧的配对样本接种到含有选择性培养基的改良琼脂平板上，并在37℃厌氧培养48小时。在检查了76对唾液样本后，发现压舌板两侧的变形链球菌计数无显著差异，这表明采样技术是合适的。方差分析显示，在四个采样时间内，每位受试者内部存在显著差异（P<0.01）。在受试者的每日平均值之间发现了显著差异（P<0.05）。
