Kuo B S, Mandagere A, Osborne D R, Hwang K K
Metabolism Division, Marion Merrell Dow Inc., Kansas City, Missouri 64134.
Pharm Res. 1990 Dec;7(12):1257-61. doi: 10.1023/a:1015933820971.
A fully automated HPLC assay for hydrochlorothiazide in plasma has been developed using a column-switching technique. The method involves direct injection of plasma to the extraction column for sample cleanup followed by switching onto the analytical column. Good precision, accuracy, and linearity were obtained over a range of 25 to 2000 ng/ml in rat, dog, and human plasma. The column-switching method has also been validated by comparison with a conventional HPLC method requiring a cumbersome plasma extraction procedure. Since the method is simple, rapid, and reproducible, it is useful for determination of hydrochlorothiazide levels in animal and human plasma.
已开发出一种使用柱切换技术测定血浆中氢氯噻嗪的全自动高效液相色谱（HPLC）分析法。该方法包括将血浆直接注入萃取柱进行样品净化，然后切换至分析柱。在大鼠、犬和人血浆中，浓度范围为25至2000 ng/ml时，该方法具有良好的精密度、准确度和线性。通过与需要繁琐血浆萃取程序的传统HPLC方法进行比较，柱切换法也得到了验证。由于该方法简单、快速且可重复，因此可用于测定动物和人血浆中的氢氯噻嗪水平。
