International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.
Microbes Infect. 2011 Jan;13(1):58-64. doi: 10.1016/j.micinf.2010.10.001. Epub 2010 Oct 16.
Human immunodeficiency virus type 1 (HIV-1) hardly replicates in Old World monkeys. Recently, a mutant HIV-1 clone, NL-DT5R, in which a small part of gag and the entire vif gene are replaced with SIVmac239-derived ones, was shown to be able to replicate in pigtail monkeys but not in rhesus monkeys (RM). In the present study, we found that a modified monkey-tropic HIV-1 (HIV-1mt), MN4-5S, acquired the ability to replicate efficiently in cynomolgus monkeys as compared with the NL-DT5R, while neither NL-DT5R nor MN4-5S replicated in RM cells. These results suggest that multiple determinants may be involved in the restriction of HIV-1 replication in macaques, depending on the species of macaques. The new HIV-1mt clone will be useful for studying molecular mechanisms by which anti-viral host factors regulate HIV-1 replication in macaques.
人类免疫缺陷病毒 1 型（HIV-1）在旧世界猴中几乎无法复制。最近，一种突变的 HIV-1 克隆 NL-DT5R，其 gag 的一小部分和整个 vif 基因被 SIVmac239 衍生的基因所取代，被证明能够在猪尾猴中复制，但不能在恒河猴（RM）中复制。在本研究中，我们发现一种改良的猴嗜性 HIV-1（HIV-1mt）MN4-5S，与 NL-DT5R 相比，能够在食蟹猴中高效复制，而 NL-DT5R 和 MN4-5S 均不能在 RM 细胞中复制。这些结果表明，多种决定因素可能参与了 HIV-1 在猕猴中的复制限制，具体取决于猕猴的种类。新的 HIV-1mt 克隆将有助于研究抗病毒宿主因子调节 HIV-1 在猕猴中复制的分子机制。
