Department of Clinical Genetics, VU University Medical Center Amsterdam, The Netherlands.
Neuron. 2010 Oct 21;68(2):207-17. doi: 10.1016/j.neuron.2010.10.010.
Neuronal development, function, and the subsequent degeneration of the brain are still an enigma in both the normal and pathologic states, and there is an urgent need to find better targets for developing therapeutic intervention. Current techniques to deconstruct the architecture of brain and disease-related pathways are best suited for following up on single genes but would take an impractical amount of time for the leads from the current wave of genetic and genomic data. New technical developments have made combined high-throughput-high-content (HT-HC) cellular screens possible, which have the potential to contextualize the information, gathered from a combination of genetic and genomic approaches, into networks and functional biology and can be utilized for the identification of therapeutic targets. Herein we discuss the potential impact of HT-HC cellular screens on medical neuroscience.
神经元的发育、功能以及随后的大脑退化在正常和病理状态下仍然是一个谜，因此迫切需要寻找更好的靶点来开发治疗干预措施。目前用于解构大脑结构和疾病相关途径的技术最适合于跟踪单个基因，但对于当前遗传和基因组数据浪潮产生的线索来说，所需时间将不切实际。新的技术发展使得高通量高内涵（HT-HC）细胞筛选成为可能，这有可能将遗传和基因组方法相结合所获得的信息纳入网络和功能生物学中，并可用于鉴定治疗靶点。本文讨论了 HT-HC 细胞筛选对医学神经科学的潜在影响。
