Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum 695 019, Kerala, India.
Arch Microbiol. 2010 Dec;192(12):1049-57. doi: 10.1007/s00203-010-0636-y. Epub 2010 Oct 19.
Exopolysaccharides (EPS) from lactic acid bacteria contribute to specific rheology and texture of fermented milk products and finds applications even in non-dairy foods and in therapeutics. Box-Behnken model of response surface methodology (RSM) was employed to formulate the production medium for exopolysaccharide (EPS). FT-IR spectral analysis of the purified EPS from Lactobacillus plantarum MTCC 9510 revealed prominent characteristic groups corresponding to polyhydric alcohols. The degradation temperature (Td) of the polysaccharide was found to be 260°C with the help of thermo gravimetric analysis (TGA). Structure elucidation of the EPS showed that it consists of a trisaccharide repeating unit of α-D: -glucose, β-D: -glucose and α-D: -mannose.
乳酸菌胞外多糖（EPS）有助于发酵乳制品的特定流变学和质地特性，并在非乳制品食品和治疗学中得到应用。采用响应面法（RSM）的 Box-Behnken 模型来制定生产培养基用于胞外多糖（EPS）。从植物乳杆菌 MTCC 9510 中提取的 EPS 的傅里叶变换红外光谱（FT-IR）分析显示，相应的多元醇特征基团显著。借助热重分析（TGA）发现多糖的降解温度（Td）为 260°C。EPS 的结构阐明表明，它由 α-D: -葡萄糖、β-D: -葡萄糖和 α-D: -甘露糖的三糖重复单元组成。
