植物乳杆菌 BC-25 胞外多糖的生产、纯化及结构研究。
Production, purification and structural study of an exopolysaccharide from Lactobacillus plantarum BC-25.
机构信息
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
College of Food Science, Sichuan Agricultural University, Ya'an 625014, China.
出版信息
Carbohydr Polym. 2016 Jun 25;144:205-14. doi: 10.1016/j.carbpol.2016.02.067. Epub 2016 Feb 26.
The exopolysaccharides (EPS) produced by Se-enriched Lactobacillus plantarum BC-25 was purified to illustrate its structure and conformational characterization. The yield of EPS (324.80mg/l) was obtained with a sodium selenite concentration of 6μg/ml. The results indicated that the EPS was soluble in water, but insoluble in organic solvents. The molecular weight of this highly thermal stability EPS was 1.83×10(4)Da and 1.33×10(4)Da with or without Se enriched respectively. The EPS was composed of mannose, galactose and glucose in a molar ratio of 92.21:1.79:6.00 and 91.36:2.44:6.20 with or without Se. This compound had a backbone of (1→2)-linked Man, (1→2.6)-linked Glc, (2→6)-linked Man, and (2→6)-linkedGal confirmed by GC-MS. IR analysis suggested that the EPS belonged to heteropolysaccharide with a pyran group, with possible presence of SeO and CSeC residues that Se may substitutes CH3 in -OCH3 in the polysaccharide as confirmed by NMR spectroscopy.
富硒植物乳杆菌 BC-25 产生的胞外多糖（EPS）被纯化以阐明其结构和构象特征。在亚硒酸钠浓度为 6μg/ml 时，EPS（324.80mg/l）的产率最高。结果表明，EPS 可溶于水，但不溶于有机溶剂。这种高度热稳定的 EPS 的分子量分别为 1.83×10(4)Da 和 1.33×10(4)Da，其中 Se 含量分别为富硒和非富硒。EPS 由甘露糖、半乳糖和葡萄糖组成，摩尔比分别为 92.21:1.79:6.00 和 91.36:2.44:6.20，富硒和非富硒。通过 GC-MS 证实，该化合物的糖链骨架由（1→2）-连接的甘露糖、（1→2.6）-连接的葡萄糖、（2→6）-连接的甘露糖和（2→6）-连接的半乳糖组成。IR 分析表明，EPS 属于吡喃糖，可能存在 SeO 和 CSeC 残基，这表明 Se 可能取代多糖中-OCH3 上的 CH3，这一点也得到了 NMR 光谱的证实。
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