Department of Surgery, University of Connecticut Health Center, MC 3105, Farmington, Connecticut 06030, USA.
J Orthop Res. 2011 Apr;29(4):609-16. doi: 10.1002/jor.21251. Epub 2010 Oct 18.
Porous tantalum (Ta) implants have been successful in various orthopedic procedures for patients with compromised bone-forming abilities. Previous studies demonstrated that human osteoblast (HOB) cultures from older female patients produced less bone on implant materials in vitro compared to HOBs from age-matched male and younger female patients. In this study, the responses of HOBs from younger (< 45) and older (> 60 years old) female patients were compared on Ta, titanium fiber mesh (TFM) and tissue culture plastic. Adhesion, proliferation, and mineralization were greater in cells from younger patients than from older patients. Cell adhesion was slightly higher on Ta than TFM or plastic. However, Ta highly stimulated cell proliferation with a 4- and 6-fold increase compared to TFM for cells from younger and older patients, respectively, and 12- and 16-fold increase in proliferation compared to cells on plastic (p ≤ 0.001). At 3 weeks, mineralization was significantly higher on Ta compared to TFM for HOBs from older patients (p ≤ 0.05). Expression levels of bone matrix markers demonstrated differences dependent on age and substrate. Scanning electron micrographs revealed HOBs covering the surfaces and entering the pores of both Ta and TFM. In conclusion, tantalum greatly stimulates cell proliferation, and improves the ability of HOBs from older patients to form bone.
多孔钽（Ta）植入物已成功应用于各种骨科手术中，适用于成骨能力受损的患者。先前的研究表明，与年龄匹配的男性和年轻女性患者的成骨细胞（HOB）相比，来自老年女性患者的 HOB 在体外培养时在植入材料上形成的骨量较少。在这项研究中，比较了年轻（<45 岁）和老年（>60 岁）女性患者的 HOB 在 Ta、钛纤维网（TFM）和组织培养塑料上的反应。与老年患者相比，年轻患者的细胞黏附、增殖和矿化程度更高。细胞黏附在 Ta 上略高于 TFM 或塑料。然而，与 TFM 相比，Ta 可分别显著刺激年轻和老年患者的细胞增殖，增殖倍数分别为 4 倍和 6 倍，与塑料上的细胞相比，增殖倍数分别增加 12 倍和 16 倍（p≤0.001）。3 周时，与 TFM 相比，老年患者的 HOB 在 Ta 上的矿化程度显著更高（p≤0.05）。骨基质标志物的表达水平因年龄和基质而异。扫描电子显微镜显示 HOB 覆盖 Ta 和 TFM 的表面并进入其孔隙。总之，钽可极大地刺激细胞增殖，并提高老年患者的 HOB 形成骨的能力。
