Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA.
J Lipid Res. 2011 Feb;52(2):389-92. doi: 10.1194/jlr.D010587. Epub 2010 Oct 19.
A simple, high-yielding preparation of monolysocardiolipin (MLCL) by phospholipase A2 hydrolysis of cardiolipin (CL) in methanol on a semi-preparative scale is described. In methanol, phospholipase A2 preferentially hydrolyzes CL to MLCL. This selectivity results in ∼80% yield of MLCL. The synthesized MLCL and dilysocardiolipin were characterized by NMR and ESI-MS/MS. Only the sn-2 position of CL was hydrolyzed by phospholipase A2 in methanol.
一种简单、高产的制备单溶血磷脂酰甘油（MLCL）的方法，通过在半制备规模的甲醇中用磷脂酶 A2 水解心磷脂（CL）得到。在甲醇中，磷脂酶 A2 优先水解 CL 生成 MLCL。这种选择性导致 MLCL 的产率约为 80%。合成的 MLCL 和二溶血磷脂酰甘油通过 NMR 和 ESI-MS/MS 进行了表征。只有 CL 的 sn-2 位在甲醇中被磷脂酶 A2 水解。
