Department of Obstetrics and Gynecology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Reprod Sci. 2011 Feb;18(2):172-9. doi: 10.1177/1933719110385129. Epub 2010 Oct 19.
The newborn rat model has been developed to elucidate the mechanism and management of perinatal brain damage. Our study hypothesis is that an acetylcholine receptor agonist (carbachol) reduces hypoxia-ischemia (HI)-induced brain damage in a well-established newborn rat model.
7-day-old Wistar rats were divided into 3 groups at random: carbachol preinjection and HI (Carb/HI), saline preinjection and HI (Saline/HI), and only HI (HI). Rats were subjected to left carotid artery ligation followed by 2 hours of hypoxia (8% oxygen). We injected carbachol or saline before hypoxic loading. After 7 days, we checked for brain damage.
In the cerebral cortex, 25% of the Carb/HI group showed mild neural damage, and the remaining 75% showed no damage. In contrast, more than 80% of the Saline/HI and HI groups had severe neural damage. Similarly, neural damage significantly decreased in Carb/HI compared with Saline/HI and HI for CA1, CA2, CA3, and the dentate gyrus of hippocampal regions.
Acetylcholine receptor agonist has a potent effect by reducing perinatal brain damage induced by HI in newborn rats.
已建立新生大鼠模型以阐明围产期脑损伤的机制和处理方法。我们的研究假设是，乙酰胆碱受体激动剂（卡巴胆碱）可减轻既定新生大鼠模型中的缺氧缺血（HI）诱导的脑损伤。
将 7 日龄 Wistar 大鼠随机分为 3 组：卡巴胆碱预处理加 HI（Carb/HI）、生理盐水预处理加 HI（Saline/HI）和仅 HI（HI）。大鼠接受左颈总动脉结扎，随后进行 2 小时缺氧（8%氧气）。在缺氧负荷前注射卡巴胆碱或生理盐水。7 天后，检查脑损伤情况。
在大脑皮层中，25%的 Carb/HI 组表现出轻度神经损伤，其余 75%无损伤。相比之下，超过 80%的 Saline/HI 和 HI 组有严重的神经损伤。同样，与 Saline/HI 和 HI 相比，Carb/HI 组的 CA1、CA2、CA3 和海马齿状回的神经损伤显著减少。
乙酰胆碱受体激动剂通过减轻新生大鼠 HI 诱导的围产期脑损伤具有显著效果。
