Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
Reprod Sci. 2011 Feb;18(2):190-9. doi: 10.1177/1933719110385133. Epub 2010 Oct 19.
Mechanisms mediating preeclampsia (PE) are unclear. Endogenous digitalis-like factors (EDLFs) are sodium pump (SP) inhibitors implicated in essential hypertension, but not fully explored in PE. This study asks whether EDLFs are present and increased in PE and considers their source.
EDLF in sera and placentas from third trimester women with uncomplicated pregnancies or PE was assessed by a Rb(+) uptake assay. A digoxin antibody Fab fragment (Digibind) known to inactivate EDLFs was also used to assess EDLFs.
PE serum caused significantly more SP inhibition than serum from uncomplicated pregnancies. This inhibition was concentration-dependent and reversed by Digibind. Serum from uncomplicated pregnancies showed no concentration-dependence or reversal with Digibind. Placental homogenates from control women showed little SP inhibition, but homogenates from PE women showed marked SP inhibition reversed by Digibind.
These studies evidence EDLF in PE serum. Additionally, PE placentas have high EDLF and may represent a source.
先兆子痫（PE）的发病机制尚不清楚。内源性洋地黄样因子（EDLFs）是钠泵（SP）抑制剂，与原发性高血压有关，但在 PE 中尚未充分研究。本研究旨在探讨 EDLFs 是否存在于 PE 中，并探讨其来源。
采用放射性铷（Rb+）摄取法检测第三孕期无并发症妊娠或 PE 妇女血清和胎盘中的 EDLF。还使用已知能使 EDLFs 失活的洋地黄抗体 Fab 片段（Digibind）来评估 EDLF。
PE 血清引起的 SP 抑制作用明显大于无并发症妊娠血清。这种抑制作用呈浓度依赖性，并可被 Digibind 逆转。无并发症妊娠血清无浓度依赖性或 Digibind 逆转。来自对照组妇女的胎盘匀浆仅显示出少量的 SP 抑制，但来自 PE 妇女的胎盘匀浆显示出明显的 SP 抑制，可被 Digibind 逆转。
这些研究证明了 PE 血清中存在 EDLF。此外，PE 胎盘具有高 EDLF，可能是其来源。
