Dept of Pathology, Oregon Health & Science University, Mail Code L-471, 3181 SW Sam Jackson Park Rd, Portland, OR 97239-3098, USA.
Am J Clin Pathol. 2010 Nov;134(5):739-48. doi: 10.1309/AJCP7LRRLK8SLUGE.
In mycosis fungoides (MF) with blood involvement, T-cell immunophenotypes in skin and blood have not been compared. Our aim was to evaluate T-cell immunophenotypes in skin by immunohistochemical analysis and compare results with flow cytometric (FC) findings in blood. Of 20 patients with MF with blood involvement, the immunophenotype was discrepant in 11 (55%). Compared with FC findings in blood, immunohistochemical analysis of skin samples failed to detect partial deletion of CD2 (5/11 [45%]), CD3 (3/11 [27%]), and CD5 (3/11 [27%]) and overrepresented deletion of CD7 in 2 (18%) of 11 patients. In addition, CD8+ MF was missed by immunohistochemical analysis in 2 (18%) of 11 patients. Identical T-cell populations were demonstrated by T-cell gene polymerase chain reaction in skin and blood in 8 of the 11 patients who had a discrepant immunophenotype. Awareness of the limitations of immunohistochemical analysis of skin samples is of practical value for pathologists interpreting skin biopsies in MF patients. In addition, our findings suggest CD8+ MF to be more common than previously reported.
在伴有血液受累的蕈样肉芽肿（MF）中，皮肤和血液中的 T 细胞免疫表型尚未进行比较。我们的目的是通过免疫组织化学分析评估皮肤中的 T 细胞免疫表型，并将结果与血液中的流式细胞术（FC）发现进行比较。在 20 例伴有血液受累的 MF 患者中，11 例（55%）存在免疫表型不一致。与血液中的 FC 发现相比，皮肤样本的免疫组织化学分析未能检测到 CD2（5/11 [45%]）、CD3（3/11 [27%]）和 CD5（3/11 [27%]）的部分缺失，以及 11 例患者中有 2 例（18%）的 CD7 过表达缺失。此外，11 例患者中有 2 例（18%）的 CD8+MF 被免疫组织化学分析漏诊。在免疫表型不一致的 11 例患者中的 8 例中，通过皮肤和血液中的 T 细胞基因聚合酶链反应证实存在相同的 T 细胞群体。了解皮肤样本免疫组织化学分析的局限性对解释 MF 患者皮肤活检的病理学家具有实际价值。此外，我们的发现表明 CD8+MF 比以前报道的更为常见。
