Carmo-Silva A Elizabete, Barta Csengele, Salvucci Michael E
U.S. Department of Agriculture, Arid-Land Agricultural Research Center, Maricopa, AZ, USA.
Methods Mol Biol. 2011;684:339-47. doi: 10.1007/978-1-60761-925-3_26.
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is a multifunctional enzyme that catalyzes the fixation of CO2 and O2 in photosynthesis and photorespiration, respectively. As the rate-limiting step in photosynthesis, improving the catalytic properties of Rubisco has long been viewed as a viable strategy for increasing plant productivity. Advances in biotechnology have made this goal more attainable by making it possible to modify Rubisco in planta. To properly evaluate the properties of Rubisco, it is necessary to isolate the enzyme in pure form. This chapter describes procedures for rapid and efficient purification of Rubisco from leaves of several species.
核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）是一种多功能酶，分别在光合作用和光呼吸过程中催化二氧化碳和氧气的固定。作为光合作用中的限速步骤，长期以来，提高Rubisco的催化特性一直被视为提高植物生产力的可行策略。生物技术的进步使得在植物体内对Rubisco进行改造成为可能，从而使这一目标更易于实现。为了准确评估Rubisco的特性，有必要以纯形式分离该酶。本章介绍了从几种植物叶片中快速高效纯化Rubisco的方法。
