从木质腐朽的珊瑚菌科（Daldinia 种）中筛选新型纤维素酶并优化其生产
Novel Cellulase Screening and Optimal Production from the Wood Decaying Xylariaceae: Daldinia Species.
机构信息
Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Department of Biotechnology, Southern Taiwan University, 1 Nan-Tai Street, Yung Kang City, Tainan County, 71005, Taiwan.
出版信息
Appl Biochem Biotechnol. 2024 Jun;196(6):1-11. doi: 10.1007/s12010-010-9102-1. Epub 2010 Oct 21.
The highest cellulases production from Daldinia caldariorum 263 (D-263) was found among Daldinia eschscholzii and Daldinia childiae. Three cellulases, one xylanase and one β-glucosidase of the molecular weights 55, 43, 34, 30, and 105 kDa, respectively, were determined by zymographic sodium dodecyl sulfate polyacrylamide gel electrophoresis. From the N-terminal sequencing, the major cellulase CelA belonging to glycosyl hydrolase family 5 was determined. By following an orthogonal experiment design (L9), factors affecting the cultivation of D. caldariorum 263 are ranked as medium composition > temperature > pH ≥ FP (%). The optimum cultivation conditions for obtaining the best FPase (600 mU/ml) at 72 h are 150 rpm, 35 °C, pH 7, 0.2% soy peptone and 0.5% α-cellulose in minimal requirement medium. In comparison with Trichoderma reesei (ATCC26921) secreting 1,135 mU/ml of FPase after 6 days cultivation at pH 5, D. caldariorum 263 grew faster at 35 °C and produced the maximum FPase within 3 days at pH 7.
从 Daldinia eschscholzii 和 Daldinia childiae 中发现了最高的纤维素酶产量来自 Daldinia caldariorum 263（D-263）。通过凝胶电泳，确定了三种纤维素酶、一种木聚糖酶和一种β-葡萄糖苷酶，分子量分别为 55、43、34、30 和 105 kDa。通过 N 端测序，确定了主要的纤维素酶 CelA 属于糖苷水解酶家族 5。通过正交实验设计（L9），对影响 D. caldariorum 263 培养的因素进行了排序，培养基组成>温度>pH 值≥FP（%）。在最小需求培养基中，获得最佳 FPase（600 mU/ml）的最佳培养条件为 150 rpm、35°C、pH 7、0.2%大豆蛋白胨和 0.5%α-纤维素，最佳培养时间为 72 小时。与在 pH 5 下培养 6 天后分泌 1,135 mU/ml FPase 的 Trichoderma reesei（ATCC26921）相比，D. caldariorum 263 在 35°C 下生长更快，在 pH 7 下 3 天内达到最大 FPase 产量。
Zotero 插件