Ehrin E, Karlberg A T
Apoteksbolaget AB, Central Laboratory, Stockholm, Sweden.
Contact Dermatitis. 1990 Nov;23(5):359-66. doi: 10.1111/j.1600-0536.1990.tb05172.x.
A method for detection of rosin (colophony) in technical products has been developed. The technique, using HPLC, is based on the analysis of abietic acid and dehydroabietic acid, major components of rosin. In the procedure described in this paper, the limit of detection was 0.001% for abietic acid and 0.015% for dehydroabietic acid. An average recovery of 88.9% for abietic acid and 88.2% for dehydroabietic acid was obtained. The lowest detectable amount of abietic acid (0.001%) corresponds to a content of 0.003% unmodified rosin in the product, assuming that the resin acid content in rosin is 90%, of which 30-50% is abietic acid. This is an acceptable limit compared to the reactivity in patients with known allergy to rosin. Technical products for analysis were supplied by different Swedish manufacturers, who also gave the approximate content of rosin in the various products. The content of rosin determined in the products was in accordance with the contents given by the manufacturers.
已开发出一种检测工业产品中松香（松脂）的方法。该技术采用高效液相色谱法（HPLC），基于对松香主要成分枞酸和脱氢枞酸的分析。在本文所述的方法中，枞酸的检测限为0.001%，脱氢枞酸的检测限为0.015%。枞酸的平均回收率为88.9%，脱氢枞酸的平均回收率为88.2%。假设松香中的树脂酸含量为90%，其中30 - 50%为枞酸，那么最低可检测量的枞酸（0.001%）相当于产品中未改性松香含量为0.003%。与已知对松香过敏患者的反应性相比，这是一个可接受的限度。用于分析的工业产品由不同的瑞典制造商提供，他们还给出了各种产品中松香的大致含量。产品中测定的松香含量与制造商给出的含量一致。
