Sadhra S, Foulds I S, Gray C N
Institute of Occupational Health, University of Birmingham, Edgbaston, U.K.
Br J Dermatol. 1996 Apr;134(4):662-8. doi: 10.1111/j.1365-2133.1996.tb06966.x.
In order to investigate the contact allergens in the unmodified colophony (rosin) used in routine patch testing, preparative chromatographic techniques were used to separate its components which were then patch tested on colophony-sensitive individuals. The chemical structure of the dermatologically active components was elucidated using mass spectrometry, nuclear magnetic resonance and infra-red techniques. The study shows that oxidized resin acids are stronger sensitizers than the resin acids themselves, the most potent contact allergen being 7-oxydehydroabietic acid (purity 92%). At naturally occurring concentrations (relative to abietic acid) all unoxidized resin acids were found to play an insignificant part, individually, in colophony dermatitis, except abietic acid (purity 99%), which was found to be a weak but important allergen. The commercial Trolab abietic acid preparation (unknown purity) was found to be dermatologically more active than purified abietic acid.
为了研究常规斑贴试验中使用的未改性松香中的接触性变应原，采用制备色谱技术分离其成分，然后在对松香敏感的个体上进行斑贴试验。使用质谱、核磁共振和红外技术阐明了具有皮肤活性成分的化学结构。该研究表明，氧化树脂酸比树脂酸本身是更强的致敏剂，最有效的接触性变应原是7-氧化脱氢枞酸（纯度92%）。在天然存在的浓度下（相对于枞酸），发现所有未氧化的树脂酸单独在松香性皮炎中所起的作用微不足道，但纯度为99%的枞酸是一种弱但重要的变应原。发现市售的Trolab枞酸制剂（纯度未知）在皮肤学上比纯化的枞酸更具活性。
