Wang Jin-Rong, Wang Yong-Zhi, Dong Fu-Hui, Zhong Hong-Gang, Wang De-Long, Wang Xuan
Department of Orthopaedics, Eye Hospital of China Academy of Chinese Medical Science, Beijing 100040, China.
Zhongguo Gu Shang. 2010 Sep;23(9):692-5.
To study the mechanism of synthesis of substance P (SP) in the dorsal root ganglion (DRG) and the release of it in the dorsal horn of the spinal cord of rats after compression of skeletal muscle, and to observe the influence of small needle knife.
Sustained pressure of 70 kPa was applied to rats, muscular tissues for 2 hours. The rats were divided into three groups: normal, control and experiment group respectively. In all rats except the six normal ones, the lower legs were compressed once one day. The left leg was considered as the control group, the right left was experiment group, which were divided into the 1st day, the 2nd day and the 3rd day within the two groups. Experiment group was treated with small needle knife after the muscular tissue was compressed. After completing the stimulation, the DRG related to the muscle and part of spinal cord were removed for the qualification of SP-like immunoreactivity using immunohistochemistry. The dark brown stains on the DRG and on the REXed laminae I and II in the dorsal horn of the spinal cord were counted by Image-Pro Plus software.
SP-like immunoreactivity in the side treated by the small needle knife was enhanced comparing with the counterpart in DRG in normal group (P < 0.01). The integrated optical density of SP like immunoreactivity of the DRG in the experiment group were significantly reduced compared with the control group (P < 0.05). However, the release of SP from spinal cord in experiment group was lower than that in the control group at the 1st day and the 3rd day (P < 0.01), with the opposite result of the 2nd day.
Based on the fact that SP is a nociceptive neurotransmitter, the present study suggests that tension relaxation by small needle knife reduces expression of SP in the DRG, and shows no effects on the release of SP from the spinal cord in short-term (3 days).
研究骨骼肌受压后大鼠背根神经节（DRG）中P物质（SP）的合成机制及其在脊髓背角的释放情况，并观察小针刀的影响。
对大鼠的肌肉组织施加70 kPa的持续压力2小时。将大鼠分为三组：正常组、对照组和实验组。除6只正常大鼠外，其余大鼠每天对小腿进行一次压迫。左腿为对照组，右腿为实验组，两组再各自分为第1天、第2天和第3天。实验组在肌肉组织受压后用小针刀治疗。刺激结束后，取出与肌肉相关的DRG和部分脊髓，采用免疫组织化学法鉴定SP样免疫反应性。使用Image-Pro Plus软件对DRG以及脊髓背角I层和II层（ Rexed分层）上的深棕色染色进行计数。
与正常组DRG中的对应部位相比，小针刀治疗侧的SP样免疫反应性增强（P < 0.01）。实验组DRG中SP样免疫反应性的积分光密度与对照组相比显著降低（P < 0.05）。然而，实验组脊髓中SP在第1天和第3天的释放低于对照组（P < 0.01），第2天结果相反。
基于SP是一种伤害性神经递质这一事实，本研究表明小针刀松解张力可降低DRG中SP的表达，且在短期内（3天）对脊髓中SP的释放无影响。
