人动脉粥样硬化斑块中对氧磷酶-1 和 3 的免疫组化分析。

Immunohistochemical analysis of paraoxonases-1 and 3 in human atheromatous plaques.

机构信息

Centre de Recerca Biomèdica, Hospital Universitari de Sant Joan, Institut d'Investigacions Sanitàries Pere Virgili, Universitat Rovira i Virgili, Reus, Catalunya, Spain.

出版信息

Eur J Clin Invest. 2011 Mar;41(3):308-14. doi: 10.1111/j.1365-2362.2010.02411.x. Epub 2010 Oct 21.

DOI:10.1111/j.1365-2362.2010.02411.x

PMID:20964682

Abstract

BACKGROUND

The paraoxonase (PON) enzyme family comprising PON1, PON2 and PON3 are antioxidant enzymes that degrade bioactive oxidised lipids and are thus antiatherogenic.

MATERIALS AND METHODS

We investigated the localisation of the PON proteins during the development of atherosclerosis by immunohistochemical analysis.

RESULTS

In normal aortas, PON1 and PON3 were localised to smooth muscle cells (SMC) and endothelial cells. PON3 staining was stronger than that of PON1. During atherosclerosis development, SMC staining for PON1 and PON3 was greatly reduced, while macrophage staining for both proteins increased with PON1 predominating. Macrophage staining for PON1 and PON3 was significantly and positively related to the amount of aortic inflammation (both P<0·001).

CONCLUSIONS

Our data add support to the growing body of evidence for a cellular protective effect of PON1 and PON3 against the proinflammatory/proatherosclerotic effects of lipid peroxidation.

摘要

背景

对氧磷酶（PON）酶家族包括 PON1、PON2 和 PON3，是抗氧化酶，可降解生物活性氧化脂质，从而具有抗动脉粥样硬化作用。

材料和方法

我们通过免疫组织化学分析研究了 PON 蛋白在动脉粥样硬化发展过程中的定位。

结果

在正常主动脉中，PON1 和 PON3 定位于平滑肌细胞（SMC）和内皮细胞。PON3 的染色强度强于 PON1。在动脉粥样硬化发展过程中，PON1 和 PON3 的 SMC 染色大大减少，而两种蛋白质的巨噬细胞染色增加，其中 PON1 占优势。PON1 和 PON3 的巨噬细胞染色与主动脉炎症的程度呈显著正相关（均 P<0·001）。