Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, Council of Scientific and Industrial Research, Lucknow 226015, India.
J Pharm Biomed Anal. 2011 Feb 20;54(3):497-502. doi: 10.1016/j.jpba.2010.09.025. Epub 2010 Sep 29.
A new high-performance thin-layer chromatographic (HPTLC) method has been developed for the simultaneous quantitation of three bioactive steroidal glycoalkaloid (SGA) markers, solasonine (SN), solamargine (SM) and khasianine (KN) in the plant Solanum xanthocarpum. Extraction efficiency of targeted SGAs from plant matrix using methanol and acidified methanol were studied using percolation, ultrasonication and microwave techniques. The separation was achieved on silica gel 60F(254) TLC plates using chloroform-methanol-water as mobile phase. The quantitation of SGAs was carried out using the densitometric reflection/absorption mode at 520 nm after post chromatographic derivatization using Dragendorff's reagent. The method was validated for peak purity, precision, accuracy, robustness, limit of detection (LOD) and quantitation (LOQ). Method specificity was confirmed using retention factor (R(f)), Vis spectral correlation and electrospray ionization mass spectra (ESI-MS) of marker compounds in the sample track.
一种新的高效薄层色谱（HPTLC）方法已经被开发出来，用于同时定量测定植物龙葵中的三种生物活性甾体糖苷生物碱（SGA）标记物，即茄碱（SN）、澳洲茄边碱（SM）和澳洲茄碱（KN）。使用甲醇和酸化甲醇通过渗滤、超声和微波技术研究了目标 SGA 从植物基质中的提取效率。在硅胶 60F（254）TLC 板上，使用氯仿-甲醇-水作为流动相进行分离。SGAs 的定量是在色谱后使用 Dragendorff 试剂进行衍生化后，在 520nm 处采用反射/吸收密度法进行的。该方法经过峰纯度、精密度、准确度、稳健性、检测限（LOD）和定量限（LOQ）验证。使用保留因子（R(f)）、样品谱带中标记化合物的可见光谱相关和电喷雾电离质谱（ESI-MS）确认了方法的特异性。
