一种用于定量检测茄属植物及其提取物中糖苷生物碱的反相高效液相色谱分析方法的验证。
A Validated Reverse Phase HPLC Analytical Method for Quantitation of Glycoalkaloids in Solanum lycocarpum and Its Extracts.
机构信息
Laboratório de Farmacognosia, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, s/n, 14040-903 Ribeirão Preto, SP, Brazil.
出版信息
J Anal Methods Chem. 2012;2012:947836. doi: 10.1155/2012/947836. Epub 2012 Mar 27.
Solanum lycocarpum (Solanaceae) is native to the Brazilian Cerrado. Fruits of this species contain the glycoalkaloids solasonine (SN) and solamargine (SM), which display antiparasitic and anticancer properties. A method has been developed for the extraction and HPLC-UV analysis of the SN and SM in different parts of S. lycocarpum, mainly comprising ripe and unripe fruits, leaf, and stem. This analytical method was validated and gave good detection response with linearity over a dynamic range of 0.77-1000.00 μg mL(-1) and recovery in the range of 80.92-91.71%, allowing a reliable quantitation of the target compounds. Unripe fruits displayed higher concentrations of glycoalkaloids (1.04% ± 0.01 of SN and 0.69% ± 0.00 of SM) than the ripe fruits (0.83% ± 0.02 of SN and 0.60% ± 0.01 of SM). Quantitation of glycoalkaloids in the alkaloidic extract gave 45.09% ± 1.14 of SN and 44.37% ± 0.60 of SM, respectively.
茄属植物（茄科）原产于巴西塞拉多。该物种的果实含有糖苷生物碱龙葵碱（SN）和茄碱（SM），具有抗寄生虫和抗癌特性。已经开发出一种用于提取和 HPLC-UV 分析不同部位 SN 和 SM 的方法，主要包括成熟和未成熟的果实、叶和茎。该分析方法经过验证，具有良好的检测响应，在线性范围内为 0.77-1000.00μg mL(-1)，回收率在 80.92-91.71%范围内，能够可靠地定量目标化合物。未成熟果实中的糖苷生物碱含量（SN 为 1.04%±0.01，SM 为 0.69%±0.00）高于成熟果实（SN 为 0.83%±0.02，SM 为 0.60%±0.01）。生物碱提取物中糖苷生物碱的定量分别为 SN 45.09%±1.14 和 SM 44.37%±0.60。
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