[肝癌细胞膜上唾液酸含量的变化]

[Variations of the amount of sialic acids on hepatocellular carcinoma cell membrane].

作者信息

Gong Zu-yuan, Liao Cai-xian, Wang Yu, Liao Xin-xin, Qin An-cheng, Huang Yong-ping, Liao Hui

机构信息

Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2010 Oct;30(10):2323-6.

PMID:20965836

Abstract

OBJECTIVE

To observe the change in the amount of sialic acids on hepatocellular carcinoma (HCC) cell membrane.

METHODS

Surgical specimens of HCC and liver cirrhosis tissues were obtained from 28 patients to prepare carcinoma cell and hepatocyte suspensions by collagenase digestion. For assay of α2, 3 and α2, 6-sialic acids, the cells were suspended in the staining buffer containing either fluorescein isothiocyanate-Maackia amurensis lectin (FITC-MAL) or fluorescein isothiocyanate-Sambucus nigra bark lectin (FITC-SNA) and incubated for 1 h, respectively. Flow cytometric analysis was carried out to measure the mean fluorescence intensity (MFI) on the cell surface.

RESULTS

In both FITC-MAL- and FITC-SNA-incubated HCC cells, the MFI on the cell surface was greater than that of the hepatocytes.

CONCLUSION

Both of α2, 3 and α2, 6- sialic acids increases significantly on the hepatocyte membrane after the carcinomatous change.

摘要

目的

观察肝癌（HCC）细胞膜上唾液酸含量的变化。

方法

从28例患者获取HCC手术标本及肝硬化组织，经胶原酶消化制备癌细胞和肝细胞悬液。为检测α2,3和α2,6 - 唾液酸，将细胞分别悬浮于含异硫氰酸荧光素 - 黑果腺肋花楸凝集素（FITC - MAL）或异硫氰酸荧光素 - 接骨木果凝集素（FITC - SNA）的染色缓冲液中，孵育1小时。进行流式细胞术分析以测量细胞表面的平均荧光强度（MFI）。