Luzzati A L, Giacomini E, Torosantucci A, Giordani L, Cassone A
Laboratory of Immunology, Istituto Superiore di Sanità, Roma, Italy.
J Biol Regul Homeost Agents. 1990 Oct-Dec;4(4):142-9.
The effects of Candida albicans mannoproteins on the induction of a primary antibody response to a T-dependent antigen, sheep erythrocytes (SRBC), in cultures of human blood lymphocytes, were investigated. Two experimental systems (bulk and limiting dilution cultures) allowing the detection of both enhancing and inhibitory effects, were used. In bulk cultures, antigen alone elicited a small number of specific antibody forming cells, unless IL-2 was supplied. Addition of the fungal mannoprotein extract or of a purified constituent of it increased 5 to more than 10 times the specific response. When limiting dilution analysis was performed, we observed that: a) a similar number of specific precursor cells was induced by antigen and either IL-2 or mannoprotein; b) the plot of the number of seeded cells versus the log of the fraction of negative cultures was linear in antigen and IL-2 triggered cultures but constantly deviated from linearity when the candidal stimulant was added. Thus, more than one type of precursor cell was limiting in these cultures, and the immunoenhancing effect of mannoprotein may involve multiple cellular interactions.
研究了白色念珠菌甘露糖蛋白对人血淋巴细胞培养物中针对T细胞依赖性抗原绵羊红细胞（SRBC）的初次抗体反应诱导的影响。使用了两种能够检测增强和抑制作用的实验系统（大量培养和有限稀释培养）。在大量培养中，除非提供IL-2，否则单独的抗原只能诱导少量特异性抗体形成细胞。添加真菌甘露糖蛋白提取物或其纯化成分可使特异性反应增加5至10倍以上。当进行有限稀释分析时，我们观察到：a）抗原以及IL-2或甘露糖蛋白诱导的特异性前体细胞数量相似；b）在抗原和IL-2触发的培养物中，接种细胞数量与阴性培养物分数的对数之间的关系图呈线性，但添加念珠菌刺激物时则持续偏离线性。因此，在这些培养物中不止一种类型的前体细胞是有限的，甘露糖蛋白的免疫增强作用可能涉及多种细胞相互作用。
