Scaringi L, Cornacchione P, Rosati E, Fettucciari K, Rossi R, Marconi P
Department of Clinical Medicine, University of Perugia, General Hospital, Italy.
Cell Immunol. 1994 May;155(2):265-82. doi: 10.1006/cimm.1994.1121.
In a previous study we demonstrated that NK/LAK effectors are quickly induced in the peritoneal cavity of CD2F1 mice by a booster dose with inactivated Candida albicans (CA) cells or by the purified cell wall mannoprotein (MP), for a long time after CA sensitization. In this study we investigated the immunologic nature and kinetics of early events of the booster phenomenon. Intraperitoneal inoculation of CA in CD2F1 mice, 30 days after pretreatment with five doses of CA (2 x 10(7) cells/mouse) over a 2-week period (CA-5d treatment), elicited a very rapid recruitment of asialo GM1+ cells, L3T4+ cells, and Ly 2+ cells. Asialo GM1+ cells and Ly 2+ cells reached a maximum number 12 hr after the booster dose, while L3T4+ cells reached the maximum after 24 hr. The number of L3T4+ cells was about twofold greater than Ly 2+ cells at all times tested. A similar kinetic pattern was found after MP booster. In C57BL/6 mice we confirmed that CA and MP boosters induced LGL which express a NK antigen, detected by 3A4 mAb, and the activation marker CD25. The peak of non-MHC-restricted PEC cytotoxicity, which was reached 24 hr after MP or CA booster, did not correspond to the time (12 hr) for maximum number increase of asialo GM1+ cells and 3A4+ cells. Two hours after CA or MP booster in PEC there was a rapid and strong increase of IL-2 mRNA expression, which persisted at a high level 24 hr after booster. In CA-5d-pretreated mice, a persistent NK/LAK-like activity in the peritoneal cavity can be maintained by boosters with MP administered every 3 days. Such treatment, which we performed up to 15 days after CA sensitization, rendered the mice more responsive to further MP boosters. Effects of CA were not restricted to the peritoneal compartment because (a) there was a rebound of splenic NK activity about 10 days after CA-5d treatment by ip route and (b) CA given by iv route significantly increased splenic NK activity up to 15-20 days after CA-5d treatment. Recombinant human interleukin 2 (rhIL-2), given ip to mice (1000 U/mouse) in combination with CA during CA-5d treatment and with MP in the booster, strongly increased the level of peritoneal NK/LAK activity and PEC cellularity.(ABSTRACT TRUNCATED AT 400 WORDS)
在先前的一项研究中，我们证明，通过用灭活的白色念珠菌（CA）细胞进行加强剂量注射或用纯化的细胞壁甘露糖蛋白（MP）处理，可在CA致敏后的很长一段时间内，快速诱导CD2F1小鼠腹腔中的NK/LAK效应细胞。在本研究中，我们调查了加强现象早期事件的免疫学性质和动力学。在CD2F1小鼠中，经过2周期间给予五剂CA（2×10⁷个细胞/小鼠）预处理（CA-5d处理）30天后，腹腔接种CA可引起去唾液酸GM1⁺细胞、L3T4⁺细胞和Ly 2⁺细胞的非常快速的募集。去唾液酸GM1⁺细胞和Ly 2⁺细胞在加强剂量后12小时达到最大数量，而L3T4⁺细胞在24小时后达到最大值。在所有测试时间点，L3T4⁺细胞的数量比Ly 2⁺细胞大约多两倍。MP加强后也发现了类似的动力学模式。在C57BL/6小鼠中，我们证实CA和MP加强诱导了表达NK抗原（由3A4单克隆抗体检测）和活化标志物CD25的大颗粒淋巴细胞（LGL）。MP或CA加强后24小时达到的非MHC限制性腹膜渗出细胞（PEC）细胞毒性峰值，与去唾液酸GM1⁺细胞和3A4⁺细胞数量增加最大值的时间（12小时）不一致。在PEC中，CA或MP加强后两小时，IL-2 mRNA表达迅速且强烈增加，并在加强后24小时持续保持在高水平。在CA-5d预处理的小鼠中，每3天用MP加强可维持腹腔中持续的NK/LAK样活性。我们在CA致敏后长达15天进行这种处理，使小鼠对进一步的MP加强更有反应。CA的作用不限于腹腔，因为（a）通过腹腔途径进行CA-5d处理后约10天，脾脏NK活性出现反弹，并且（b）通过静脉途径给予CA在CA-5d处理后15 - 20天显著增加脾脏NK活性。在CA-5d处理期间将重组人白细胞介素2（rhIL-2）腹腔注射给小鼠（1000 U/小鼠）并在加强时与MP联合使用，可强烈增加腹腔NK/LAK活性水平和PEC细胞数量。（摘要截短为400字）
