van Beek J H, Westerhof N
Laboratory for Physiology, Free University, Amsterdam, The Netherlands.
Adv Exp Med Biol. 1990;277:415-23. doi: 10.1007/978-1-4684-8181-5_47.
We determined the speed with which mitochondrial oxygen consumption and therefore the mitochondrial ATP-synthesis adapted to changes in metabolic demand in the rabbit heart. This was done by measuring the oxygen uptake of the whole heart during a stepwise change in heart rate and correcting for the time taken by diffusion and by convective transport in the blood vessels. Data for the correction for transport time were obtained from the response of venous oxygen concentration to a stepwise change of arterial oxygen concentration. The time constant of the response of mitochondrial oxygen consumption to a step change in heart rate was found to be 4-8 s.
我们测定了兔心脏中线粒体氧消耗速率，进而测定了线粒体ATP合成适应代谢需求变化的速率。这是通过在心率逐步变化期间测量整个心脏的氧摄取量，并校正血管中扩散和对流运输所花费的时间来完成的。运输时间校正的数据是从静脉氧浓度对动脉氧浓度逐步变化的反应中获得的。发现线粒体氧消耗对心率阶跃变化反应的时间常数为4 - 8秒。
