Graduate School of Environmental Studies, Tohoku University, Aoba 6-6-20, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
Biomed Mater. 2010 Dec;5(6):065008. doi: 10.1088/1748-6041/5/6/065008. Epub 2010 Oct 22.
A polymeric percutaneous device with good biocompatibility and resistance to bacterial infection is required clinically. In this study, a fibroblast growth factor-2 (FGF-2)-hydroxyapatite (HAp) composite layer (FHAp layer) was formed on the surfaces of ethylene-vinyl alcohol copolymer (EVOH) specimens using a coating process in a supersaturated calcium phosphate solution supplemented with FGF-2. FGF-2 in the FHAp layer retained its biological activity to promote proliferation of fibroblasts. The EVOH specimens coated with HAp and FHAp layers were percutaneously implanted in the scalp of rats. Not only the HAp layer but also the FHAp layer showed good biocompatibility, and FGF-2 showed no harmful effects on the skin tissue responses to the implanted specimen as long as 14 d. No significantly higher infection resistance was verified for the FHAp layer over the HAp layer, although an FHAp layer coated on a metallic percutaneous device for bone fixation demonstrated higher resistance to bacterial infection over an HAp layer in the previous study. The efficacy of FHAp layers coated on percutaneous implants in resistance to bacterial infection depends on physical factors including fixation condition, stiffness and movement of implants.
临床上需要一种具有良好生物相容性和抗细菌感染能力的聚合物经皮器械。在这项研究中，采用涂层工艺在乙烯-乙烯醇共聚物（EVOH）标本表面形成成纤维细胞生长因子-2（FGF-2）-羟基磷灰石（HAp）复合层（FHAp 层），在补充有 FGF-2 的过饱和磷酸钙溶液中。FHAp 层中的 FGF-2 保留了其生物活性，以促进成纤维细胞的增殖。将涂有 HAp 和 FHAp 层的 EVOH 标本经皮植入大鼠头皮。HAp 层和 FHAp 层均表现出良好的生物相容性，FGF-2 对植入标本皮肤组织反应的不良影响最长可持续 14 天。尽管在前一项研究中，涂覆在用于骨固定的金属经皮器械上的 FHAp 层显示出比 HAp 层更高的抗细菌感染能力，但 FHAp 层并未显示出比 HAp 层更高的抗感染能力。涂覆在经皮植入物上的 FHAp 层的抗细菌感染能力的功效取决于物理因素，包括固定条件、植入物的刚度和运动。
