Sogo Yu, Ito Atsuo, Onoguchi Masahiro, Oyane Ayako, Tsurushima Hideo, Ichinose Noboru
National Institute of Advanced Industrial Science and Technology (AIST), Institute for Human Science and Biomedical Engineering, Central 6, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Biomed Mater. 2007 Sep;2(3):S175-80. doi: 10.1088/1748-6041/2/3/S16. Epub 2007 Jul 30.
Fibroblast growth factor-2 (FGF-2) was immobilized on a hydroxyapatite (HAP) ceramic in supersaturated calcium phosphate solution prepared using solutions corresponding to clinically approved infusion fluids. To avoid the risk of FGF-2 denaturation, FGF-2 immobilization was carried out at 25 degrees C. FGF-2 was successfully immobilized on HAP ceramic surfaces by deposition with calcium phosphate to form a FGF-2 and calcium phosphate composite layer. A maximum of 2.72 +/- 0.01 microg cm(-2) of FGF-2 was immobilized in the composite layer formed on the HAP ceramic under the optimum condition. A FGF-2-immobilized HAP ceramic is likely to have the ability to release a sufficient amount of FGF-2 to promote bone formation. FGF-2 released from a FGF-2-immobilized HAP ceramic maintained its biological activity, since the proliferation of fibroblastic NIH3T3 was promoted. Therefore, the FGF-2-immobilized HAP ceramic is expected to be a useful material for promoting new bone formation.
成纤维细胞生长因子-2（FGF-2）在使用临床认可的输液对应溶液制备的过饱和磷酸钙溶液中固定于羟基磷灰石（HAP）陶瓷上。为避免FGF-2变性风险，FGF-2固定在25摄氏度下进行。FGF-2通过与磷酸钙沉积成功固定在HAP陶瓷表面，形成FGF-2与磷酸钙复合层。在最佳条件下，HAP陶瓷上形成的复合层中最多可固定2.72±0.01微克/平方厘米的FGF-2。固定有FGF-2的HAP陶瓷可能有能力释放足够量的FGF-2以促进骨形成。从固定有FGF-2的HAP陶瓷释放的FGF-2保持其生物活性，因为成纤维细胞NIH3T3的增殖得到促进。因此，固定有FGF-2的HAP陶瓷有望成为促进新骨形成的有用材料。
