Knollmann U, Acker H, Langer H, Delpiano M A
Institut für Tierphysiologie, Ruhr-Universität Bochum, F.R.G.
Adv Exp Med Biol. 1990;277:713-7. doi: 10.1007/978-1-4684-8181-5_81.
Our experiments on the isolated and superfused crab retina reveal that pronounced gradients for PO2 and pH exist in this tissue. The PgO2 profiles and a delayed recovery of the PgO2 after hypoxia seem to be a consequence of the oxygen consumption inside the tissue, as much as both characteristics can be abolished by impairment of electron transport in the respiratory chain after application of antimycin A. The pH profile is obviously created by a production and steady release of lactate, which could be measured in the superfusate. As this lactate release is occurring inspite of a sufficient oxygen supply and consumption, it can be concluded that this tissue performs aerobic glycolysis.
我们对分离并进行灌流的蟹视网膜所做的实验表明，该组织中存在明显的氧分压（PO2）和pH梯度。PgO2分布以及缺氧后PgO2的延迟恢复似乎是组织内耗氧的结果，因为在应用抗霉素A后，呼吸链中电子传递受损可消除这两个特征。pH分布显然是由乳酸的产生和持续释放造成的，乳酸可在灌流液中检测到。由于尽管有充足的氧气供应和消耗，乳酸仍在释放，因此可以得出结论，该组织进行有氧糖酵解。
