Department of Chemical Engineering and Materials Science, University of Minnesota-Twin Cities, Minneapolis, MN 55455, USA.
Electrophoresis. 2010 Nov;31(22):3675-80. doi: 10.1002/elps.201000331. Epub 2010 Oct 21.
We provide an experimental test of the universal behavior arising in simulations of the electrophoretic collision of a long DNA molecule with an isolated, thin post. Our experiments take advantage of a robust protocol to embed matrices of circa 100 nm radius ZnO nanowires in a microfluidic channel. The density and height of the nanowires are easily controlled by the seeding and growth conditions. Single-molecule videomicroscopy of λ-DNA electrophoresis in a sparse nanowire array shows that the average holdup time decays exponentially with the impact parameter, in agreement with simulations. Only at the largest electric field studied here does the hooking probability approach that for an infinitesimally thin post.
我们提供了一个实验测试，以验证在模拟长 DNA 分子与孤立的薄柱体电泳碰撞时出现的普遍行为。我们的实验利用了一种强大的方案，即将大约 100nm 半径的 ZnO 纳米线矩阵嵌入微流道中。纳米线的密度和高度可以通过种子和生长条件来轻松控制。在稀疏纳米线阵列中对 λ-DNA 电泳的单分子视频显微镜观察表明，平均滞留时间与冲击参数呈指数衰减，与模拟结果一致。只有在所研究的最大电场下，挂钩概率才会接近无穷薄柱体的情况。
