[用于估计死后间隔时间的猪视网膜细胞DNA降解]
[DNA degradation of porcine retinal cells for estimation of postmortem interval].
作者信息
Fang Chao, Lin Xiao-hong, Jing Hua-lan
机构信息
Department of Forensic Pathology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China.
出版信息
Fa Yi Xue Za Zhi. 2010 Aug;26(4):250-2.
OBJECTIVE
To investigate DNA degradation of porcine retinal cells by single cell gel electrophoresis (SCGE) for estimation of postmortem interval (PMI).
METHODS
Degradation of retinal cells was observed by SCGE at 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22 and 24h after death respectively, under the environmental conditions of being kept in dark place as well as a controlled temperature of (15 +/- 2) degrees C and humidity of (50 +/- 5)%. The comet pictures were captured by fluorescence microscope and analyzed by single cell gel electrophoresis (IMI 1.0).
RESULTS
From 2h to 24h postmortem, the degree of degradation of retinal DNA increased with the prolongation of PMI. The postmortem regression functions of head DNA%, L(T)/L(H), I(T)/I(H) were y = 92.227-5.188 x + 0.019 x2 + 0.001 x3 (R2 = 0.971), y = 0.035e(0.191x) (R2 = 0.947), y = 0.099e(0.264x) (R2 = 0.955), respectively.
CONCLUSION
The examination of retinal cell DNA degradation by SCGE is useful for estimating PMI.
目的
通过单细胞凝胶电泳（SCGE）研究猪视网膜细胞的DNA降解情况，以估计死后间隔时间（PMI）。
方法
分别于死后2、4、6、8、10、12、14、16、18、20、22和24小时，在黑暗环境以及（15±2）℃温度和（50±5）%湿度的控制条件下，通过SCGE观察视网膜细胞的降解情况。用荧光显微镜拍摄彗星图像，并通过单细胞凝胶电泳（IMI 1.0）进行分析。
结果
死后2小时至24小时，视网膜DNA的降解程度随PMI延长而增加。头部DNA%、L(T)/L(H)、I(T)/I(H)的死后回归函数分别为y = 92.227 - 5.188x + 0.019x² + 0.001x³（R² = 0.971），y = 0.035e(0.191x)（R² = 0.947），y = 0.099e(0.264x)（R² = 0.955）。
结论
通过SCGE检测视网膜细胞DNA降解情况对估计PMI有帮助。
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