Karaduleva E V, Vikhliantsev I M, Podlubnaia Z A
Biofizika. 2010 Jul-Aug;55(4):612-8.
Changes in the expression of N2B- and N2BA-isoforms of titin in the left ventricle of the myocardium of spontaneously hypertensive rats during the development of hypertrophy have been analyzed by the methods of real-time scale polymerase chain reaction and SDS gel electrophoresis. It was shown that, in early development of hypertrophy (15-week-old rats), an increase in the expression of mRNA of the titin gene and a decrease in the content of the protein itself occur. At a later stage of development (26-week-old rats), a decrease in the expression of titin at the level of both mRNA and the protein per se was observed. The results obtained can be used in the development of methods for diagnosing the development of myocardium hypertrophy.
采用实时定量聚合酶链反应和十二烷基硫酸钠凝胶电泳方法，分析了自发性高血压大鼠心肌左心室中肌联蛋白N2B和N2BA亚型在肥厚发展过程中的表达变化。结果表明，在肥厚早期发展阶段（15周龄大鼠），肌联蛋白基因的mRNA表达增加，而蛋白质本身的含量减少。在发展后期（26周龄大鼠），观察到肌联蛋白在mRNA和蛋白质本身水平上的表达均降低。所获得的结果可用于开发诊断心肌肥厚发展的方法。
