Department of Biosciences, University of Eastern Finland, Finland.
J Proteome Res. 2010 Dec 3;9(12):6160-8. doi: 10.1021/pr100251p. Epub 2010 Oct 22.
Comparative targeted compositional analysis is currently an important element in the safety assessment of genetically modified plants. Profiling methods have been suggested as nontargeted tools to improve the detection of possible unintended effects. In this study, the capability of 2-dimensional electrophoresis to detect significant differences among seven conventional maize (Zea mays) cultivars grown in six different locations in Germany during two consecutive seasons was evaluated. Besides maize genotype, both geographic location and season had a significant effect on protein profiles. Differences as high as 55- and 53-fold in the quantity of specific proteins were recorded, the median observed difference being around 6- and 5-fold between the genotypes and growing locations, respectively. Understanding the variation in the quantity of individual proteins should help to put the variation of endogenous proteins and the novel proteins in the genetically modified plants in perspective. This together with the targeted analyses the profiling methods, including proteomics, could also help to get a deeper insight into the unintended alterations that might have occurred during the genetic modification process.
比较靶向组成分析是当前转基因植物安全评估的一个重要组成部分。 分析方法已被提议作为非靶向工具，以提高对可能的非预期影响的检测。 在这项研究中，评估了二维电泳在检测两个连续季节在德国六个不同地点生长的七种常规玉米（Zea mays）品种之间的显著差异的能力。 除了玉米基因型外，地理位置和季节对蛋白质图谱都有显著影响。 记录到特定蛋白质数量的差异高达 55- 和 53 倍，观察到的中位数差异分别在基因型和种植地点之间约为 6- 和 5 倍。 了解个别蛋白质数量的变化应该有助于正确看待转基因植物中内源性蛋白质和新蛋白质的变化。 这与靶向分析一起，包括蛋白质组学，也可以帮助更深入地了解在遗传修饰过程中可能发生的意外改变。
