Department of Otorhinolaryngology–Head and Neck Surgery, Guro Hospital, Korea University College of Medicine, Seoul, South Korea.
Am J Rhinol Allergy. 2010 Nov-Dec;24(6):127-31. doi: 10.2500/ajra.2010.24.3503. Epub 2010 Oct 21.
Extracellular matrix metalloproteinase inducer (EMMPRIN) is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily. The presence of EMMPRIN in nontumoral tissues suggests a role in other physiological and pathological situations, which may be associated with increased matrix metalloproteinase expression. The purpose of this study was to investigate the expression of EMMPRIN mRNA (OMIM *606080) and to localize the EMMPRIN protein in nasal polyps and healthy nasal mucosa.
The expression of EMMPRIN was investigated in the nasal polyps of 10 patients undergoing endonasal sinus surgery and compared with nasal mucosal samples obtained from 10 healthy controls. EMMPRIN mRNA was extracted from the tissues, and then a reverse transcription-polymerase chain reaction was performed. Western blot analysis was used to analyze differences in the levels of expression of EMMPRIN protein between patients with nasal polyps and healthy controls, and the EMMPRIN protein was localized in immunohistochemical staining and quantitative analysis of immunopositivity.
The levels of expression of EMMPRIN mRNA and protein were significantly increased in patients with nasal polyps compared with healthy controls. EMMPRIN protein was expressed in the epithelium and infiltrating inflammatory cells of nasal polyps and the healthy nasal mucosa. The percentages of the immune-stained area and the number of EMMPRIN-immunopositive inflammatory cells per millimeter were significantly elevated in nasal polyps compared with controls.
EMMPRIN is expressed in nasal mucosa and in nasal polyps, and the level of EMMPRIN expression is increased in nasal polyps. These results suggest that the increased expression of EMMPRIN may play a role in the pathogenesis of nasal polyps.
细胞外基质金属蛋白酶诱导因子（EMMPRIN）是一种跨膜糖蛋白，属于免疫球蛋白超家族。在非肿瘤组织中存在 EMMPRIN 表明其在其他生理和病理情况下发挥作用，这可能与基质金属蛋白酶表达增加有关。本研究旨在研究 EMMPRIN mRNA（OMIM *606080）的表达，并定位 EMMPRIN 蛋白在鼻息肉和健康鼻黏膜中的表达。
我们研究了 10 例接受鼻内镜鼻窦手术的鼻息肉患者和 10 例健康对照者的鼻黏膜组织中 EMMPRIN 的表达。从组织中提取 EMMPRIN mRNA，然后进行逆转录-聚合酶链反应。Western blot 分析用于分析鼻息肉患者和健康对照者之间 EMMPRIN 蛋白表达水平的差异，并通过免疫组化染色和免疫阳性率的定量分析定位 EMMPRIN 蛋白。
与健康对照组相比，鼻息肉患者的 EMMPRIN mRNA 和蛋白表达水平显著增加。EMMPRIN 蛋白在鼻息肉的上皮和浸润性炎症细胞以及健康鼻黏膜中表达。与对照组相比，鼻息肉中免疫染色面积的百分比和每毫米 EMMPRIN 免疫阳性炎症细胞数明显升高。
EMMPRIN 在内鼻黏膜和鼻息肉中表达，并且在鼻息肉中表达增加。这些结果表明，EMMPRIN 的表达增加可能在鼻息肉的发病机制中起作用。
