Department of Otorhinolaryngology-Head and Neck Surgery, Guro Hospital, Korea University College of Medicine, Seoul, South Korea.
Am J Rhinol Allergy. 2012 Jul-Aug;26(4):274-7. doi: 10.2500/ajra.2012.26.3771.
Intelectin-1 is a new type of Ca(2+)-dependant soluble lectin in humans that has affinity for galactofuranose in carbohydrate chains of bacterial cell walls, indicating that intelectin-1 may play a role in immune defense against bacteria. The purpose of the current study was to determine the expression of intelectin-1 mRNA and protein and to localize intelectin-1 protein in nasal polyps and tissues from control subjects.
Normal sphenoid sinus mucosa was obtained from 10 patients undergoing surgery for pituitary tumor. Nasal polyp samples were obtained from 10 patients undergoing endoscopic sinus surgery for chronic polypoid rhinosinusitis. Real-time polymerase chain reaction (PCR) was performed for intelectin-1 mRNA. Immunofluorescent staining was done for localization of intelectin-1 and quantitatively analyzed using computer-based image analysis. Western blot analysis was performed.
Real-time PCR and Western blot analysis showed that intelectin-1 expression in nasal polyps was increased compared with normal sinus mucosa. Using immunofluorescent staining, intelectin-1 was strongly stained in epithelium and submucosa of nasal polyps, and faint staining was found in normal sinus mucosa.
Intelectin-1 is expressed in human sinus mucosa and is increased in patients with nasal polyps. These results suggest a possible contribution for intelectin-1 in the pathophysiology of nasal polyps.
人肠上皮细胞凝集素-1（intelectin-1）是一种新型的 Ca2+依赖性可溶性凝集素，对细菌细胞壁糖链中的半乳糖呋喃糖具有亲和力，这表明 intelectin-1 可能在针对细菌的免疫防御中发挥作用。本研究旨在确定 intelectin-1 mRNA 和蛋白的表达，并定位 intelectin-1 蛋白在鼻息肉和对照组织中的位置。
从 10 例行垂体瘤手术的患者中获得正常蝶窦黏膜。从 10 例行内镜鼻窦手术治疗慢性鼻息肉鼻窦炎的患者中获得鼻息肉样本。进行实时聚合酶链反应（PCR）以检测 intelectin-1 mRNA。进行免疫荧光染色以定位 intelectin-1，并使用基于计算机的图像分析进行定量分析。进行 Western blot 分析。
实时 PCR 和 Western blot 分析显示，与正常鼻窦黏膜相比，鼻息肉中的 intelectin-1 表达增加。免疫荧光染色显示，鼻息肉的上皮和黏膜下层中 intelectin-1 染色强烈，而正常鼻窦黏膜中染色较弱。
intelectin-1 在人鼻窦黏膜中表达，并在鼻息肉患者中增加。这些结果提示 intelectin-1 可能参与鼻息肉的病理生理过程。
