National Institute of Agrobiological Sciences, Division of Insect Sciences, 1-2, Ohwashi, Tsukuba, Ibaraki 305-8634, Japan.
J Insect Physiol. 2011 Jan;57(1):147-52. doi: 10.1016/j.jinsphys.2010.10.003. Epub 2010 Oct 27.
Juvenile hormone III skipped bisepoxide (JHSB(3)), methyl (2R,3S,10R)-2,3;10,11-bisepoxyfarnesoate was recently determined as a novel juvenile hormone (JH) in a stink bug, Plautia stali. To further confirm the biological function of JHSB(3) in this insect, its juvenilizing, reproduction-stimulating and diapause-terminating activities and the presence in the hemolymph were examined. Topical application of JHSB(3) to last instar nymphs inhibited their metamorphosis in a dose-dependent fashion. In allatectomized and diapausing adults, JHSB(3) application exerted stimulatory effects on the development of ovaries and ectadenia in females and males, respectively. JHSB(3) was detected from the hemolymph of reproductively active females by gas chromatography-mass spectrometry analysis while its titer in the hemolymph collected from diapausing adults was too low to be detected. These results demonstrated that JHSB(3) has biological function as a JH in P. stali. Topical application of JHSB(3), its stereoisomers and 10R-JH III also indicated that compounds with the 2R,3S-configuration were more potent than those with the 2S,3R-configuration and 2,3-double bond.
幼虫激素 III 差向双环氧化物（JHSB(3)），（2R,3S,10R）-2,3;10,11-双环氧法呢酯甲酯最近被确定为一种新型的昆虫幼虫激素（JH），存在于臭虫 Plautia stali 中。为了进一步证实 JHSB(3)在这种昆虫中的生物学功能，研究了其促幼、促生殖和解除滞育的活性及其在血淋巴中的存在。JHSB(3)的局部应用于末龄若虫以剂量依赖的方式抑制其变态。在去神经和滞育成虫中，JHSB(3)的应用对雌、雄虫的卵巢发育和外生殖器发育分别具有刺激作用。通过气相色谱-质谱分析，从有生殖力的雌性血淋巴中检测到 JHSB(3)，而从滞育成虫收集的血淋巴中 JHSB(3)的浓度太低而无法检测到。这些结果表明 JHSB(3)在 P. stali 中具有 JH 的生物学功能。JHSB(3)、其立体异构体和 10R-JH III 的局部应用也表明，具有 2R,3S-构型的化合物比具有 2S,3R-构型和 2,3-双键的化合物更有效。
