Henan University of Traditional Chinese Medicine, Zhengzhou 450008, People's Republic of China.
J Ethnopharmacol. 2011 Jan 27;133(2):531-7. doi: 10.1016/j.jep.2010.10.028. Epub 2010 Oct 20.
The present study was designed to investigate the effects of the EtOH and H(2)O extracts of Selaginella tamariscina (Beauv.) Spring on hyperglycemia in diabetic rats and HepG2 cells, and to confirm the active fractions of EtOH extract in HepG2 cells.
HepG2 cells and type II diabetic rats induced by low-dose streptozotocin (STZ) and high-fat diet (HFD) were used to evaluate the hypoglycemic effect of EtOH and H(2)O extracts of Selaginella tamariscina. HepG2 cells were used to evaluate the promotive effect of different fractions of EtOH extract obtained from a polyamide column on glucose utilization.
The results in HepG2 cells indicated that the EtOH extract had a better hypoglycemic effect than the H(2)O extract. The results in diabetic rats indicated that both EtOH extract and H(2)O extract were able to ameliorate the fasting blood glucose (FBG) level and improve oral glucose tolerance (OGTT). Total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-c), free fatty acids (FFA), tumor necrosis factor-α (TNF-α), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN) and malondialdehyde (MDA) levels in serum were lowered. High density lipoprotein (HDL-c), insulin and superoxide dismutase (SOD) levels in serum were elevated as well as the hepatic glycogen content in diabetic rats. Compared with H(2)O extract, the effects of EtOH extract were more marked. The 80% ethanol fraction exhibited a stronger hypoglycemic effect than the aqueous and 50% ethanol fractions, but the 95% ethanol fraction did not show any appreciable effects in HepG2 cells.
The results suggested that the EtOH extract had a better hypoglycemic effect than the H(2)O extract; the 80% ethanol fraction from polyamide column had a strong hypoglycemic activity in HepG2 cells.
本研究旨在探讨卷柏（Beauv.）春醇提物和水提物对糖尿病大鼠和 HepG2 细胞高血糖的影响，并确定醇提物在 HepG2 细胞中的活性部位。
采用低剂量链脲佐菌素（STZ）和高脂饮食（HFD）诱导的 HepG2 细胞和 2 型糖尿病大鼠，评价卷柏醇提物和水提物的降血糖作用。采用 HepG2 细胞评价聚酰胺柱得到的不同醇提物部位对葡萄糖利用的促进作用。
HepG2 细胞实验结果表明，醇提物的降血糖作用优于水提物。糖尿病大鼠实验结果表明，醇提物和水提物均能改善空腹血糖（FBG）水平，改善口服葡萄糖耐量（OGTT）。血清总胆固醇（TC）、三酰甘油（TG）、低密度脂蛋白胆固醇（LDL-c）、游离脂肪酸（FFA）、肿瘤坏死因子-α（TNF-α）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、血尿素氮（BUN）和丙二醛（MDA）水平降低，血清高密度脂蛋白（HDL-c）、胰岛素和超氧化物歧化酶（SOD）水平升高，肝糖原含量增加。与水提物相比，醇提物的作用更为明显。80%乙醇部位的降血糖作用强于水部位和 50%乙醇部位，但 95%乙醇部位在 HepG2 细胞中无明显作用。
结果表明，醇提物的降血糖作用优于水提物；聚酰胺柱 80%乙醇部位在 HepG2 细胞中具有较强的降血糖活性。
