College of Agriculture and Biotechnology, China Agricultural University, Beijing, PR China.
Anal Chim Acta. 2010 Nov 8;680(1-2):26-31. doi: 10.1016/j.aca.2010.09.034. Epub 2010 Sep 25.
A preconcentration technique known as cloud point extraction was developed for the determination of trace levels of triazole fungicides tricyclazole, triadimefon, tebuconazole and diniconazole in environmental waters. The triazole fungicides were extracted and preconcentrated using polyethylene glycol 600 monooleate (PEG600MO) as a low toxic and environmentally benign nonionic surfactant, and determined by high performance liquid chromatography/ultraviolet detection (HPLC-UV). The extraction conditions were optimized for the four triazole fungicides as follows: 2.0 wt% PEG600MO, 2.5 wt% Na(2)SO(4), equilibration at 45°C for 10 min, and centrifugation at 2000 rpm (533 × g) for 5 min. The triazole fungicides were well separated on a reversed-phase kromasil ODS C(18) column (250 mm × 4.6 mm, 5 μm) with gradient elution at ambient temperature and detected at 225 nm. The calibration range was 0.05-20 μg L(-1) for tricyclazole and 0.5-20 μg L(-1) for the other three classes of analytes with the correlation coefficients over 0.9992. Preconcentration factors were higher than 60-fold for the four selected fungicides. The limits of detection were 6.8-34.5 ng L(-1) (S/N=3) and the recoveries were 82.0-96.0% with the relative standard deviations of 2.8-7.8%.
一种称为浊点萃取的预浓缩技术被开发用于测定环境水中痕量三唑类杀菌剂三环唑、三唑酮、戊唑醇和烯唑醇。使用聚乙二醇 600 单油酸酯（PEG600MO）作为低毒和环境友好的非离子表面活性剂提取和预浓缩三唑类杀菌剂，并通过高效液相色谱/紫外检测（HPLC-UV）进行测定。优化了四种三唑类杀菌剂的萃取条件如下：2.0wt%PEG600MO、2.5wt%Na2SO4、在 45°C 下平衡 10min 和在 2000rpm（533×g）下离心 5min。三唑类杀菌剂在反相 kromasil ODS C（18）柱（250mm×4.6mm，5μm）上在环境温度下进行梯度洗脱，在 225nm 处检测。三环唑的校准范围为 0.05-20μg L(-1)，其余三种类别的分析物的校准范围为 0.5-20μg L(-1)，相关系数均大于 0.9992。四种选定的杀菌剂的预浓缩因子均高于 60 倍。检测限为 6.8-34.5ng L(-1)（S/N=3），回收率为 82.0-96.0%，相对标准偏差为 2.8-7.8%。
