Department of Chemistry, Occidental College, 1600 Campus Road, Los Angeles, CA 90041, USA.
Biophys Chem. 2010 Nov;152(1-3):178-83. doi: 10.1016/j.bpc.2010.09.006. Epub 2010 Sep 29.
Differential scanning calorimetry (DSC) was used to analyze the thermotropic phase behavior of dipalmitoylphosphatidylcholine (DPPC) bilayers in the presence of pentanol isomers. The concentration of each pentanol isomer needed to induce the interdigitated phase was determined by the appearance of a biphasic effect in the main transition temperatures, the onset of a hysteresis associated with the main transition from the gel-to-liquid crystalline phase, and the disappearance of the pretransition. Lower threshold concentrations were found to correlate with isomers of greater alkyl chain length while branching of the alkyl chain was found to increase biphasic behavior. The addition of a methyl group to butanol systems drastically decreased threshold concentrations. However, as demonstrated in the DPPC/neopentanol system, branching of the alkyl chain away from the -OH group lowers the threshold concentration while maintaining a biphasic effect.
差示扫描量热法（DSC）用于分析二棕榈酰磷脂酰胆碱（DPPC）双层在戊醇异构体存在下的热致相行为。通过主转变温度出现双相效应、与凝胶到液晶相的主转变相关的滞后的开始以及预转变的消失，确定了诱导互贯相所需的每种戊醇异构体的浓度。发现较低的阈值浓度与烷基链较长的异构体相关，而烷基链的支化被发现增加了双相行为。在丁醇体系中添加甲基会大大降低阈值浓度。然而，正如在 DPPC/新戊醇体系中所证明的那样，远离-OH 基团的烷基链支化会降低阈值浓度，同时保持双相效应。
