通过优化培养基提高黑曲霉甘露聚糖酶的产量。
Improved mannan-degrading enzymes' production by Aspergillus niger through medium optimization.
机构信息
Freshwater Fisheries Research Centre, 75350 Batu Berendam, Malacca, Malaysia.
出版信息
N Biotechnol. 2011 Feb 28;28(2):146-52. doi: 10.1016/j.nbt.2010.10.008. Epub 2010 Oct 21.
The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL(-1)) was much higher than those observed on other carbon substrates, locust bean gum (1148 nkat mL(-1)), α-cellulose (10.7 nkat mL(-1)), glucose (8.8 nkat mL(-1)) and carboxymethylcellulose (4.6 nkat mL(-1)). For fermentation using GG as a carbon source, bacteriological peptone gave the highest β-mannanase activity (1744 nkat mL(-1)) followed by peptone from meat (1168 nkat mL(-1)), yeast extract (817 nkat mL(-1)), ammonium sulphate (241 nkat mL(-1)), ammonium nitrate (113 nkat mL(-1)) and ammonium chloride (99 nkat mL(-1)) when used as a nitrogen source. The composition of bacteriological peptone and initial pH of the medium were further optimized using response surface methodology (RSM). Medium consisted of 21.3 g L(-1) GG and 57 g L(-1) peptone with initial culture pH of 5.5 was optimum for β-mannanase production (2063 nkat mL(-1)) by A. niger. The β-mannanase production obtained in this study using A. niger was significantly higher than those reported in the literature.
采用摇瓶培养法研究了不同碳源和氮源对黑曲霉产甘露聚糖酶（尤其是β-甘露聚糖酶）的影响。黑曲霉在瓜尔胶（GG，1495 nkat mL(-1))上生长时获得的β-甘露聚糖酶活性远高于其他碳底物，如刺槐豆胶（1148 nkat mL(-1))、α-纤维素（10.7 nkat mL(-1))、葡萄糖（8.8 nkat mL(-1))和羧甲基纤维素（4.6 nkat mL(-1))。对于以 GG 作为碳源的发酵，细菌蛋白胨的β-甘露聚糖酶活性最高（1744 nkat mL(-1))，其次是肉蛋白胨（1168 nkat mL(-1))、酵母提取物（817 nkat mL(-1))、硫酸铵（241 nkat mL(-1))、硝酸铵（113 nkat mL(-1))和氯化铵（99 nkat mL(-1))。采用响应面法（RSM）对细菌蛋白胨的组成和培养基初始 pH 值进行了进一步优化。培养基由 21.3 g L(-1) GG 和 57 g L(-1)蛋白胨组成，初始培养 pH 值为 5.5 时，黑曲霉β-甘露聚糖酶的产量最高（2063 nkat mL(-1))。本研究中黑曲霉生产的β-甘露聚糖酶的产量明显高于文献报道的产量。
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