Cui F, Shi J, Lu Z
Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080.
Wei Sheng Wu Xue Bao. 1999 Feb;39(1):60-3.
Neutral beta-mannanase was produced by Bacillus subtilis BM9602 isolated from a soil. For this strain the neutral beta-mannanase was produced only when polysaccharides were used as a carbon source. Organic nitrogen source was superior to inorganic nitrogen source on the enzyme production. The optimum liquid medium consisted of 4% konjak powder, 1% each of beef peptone and yeast extract. The optimum culture conditions were initial pH 8.5, temperature 35 degrees C and cultivation time 36 h. Enzyme activity of culture filtrate to 0.5% galactomannan polysaccharide was 96 IU/mL per minute at pH 6.0 and 50 degrees C for 10 min. The optimum pH and temperature for enzyme action were 6.0 and 50 degrees C respectively. The enzyme was stable below 50 degrees C and pH 5.0-10.0. The enzyme hydrolyzed konjak powder and locust bean gum to form oligosaccharides.
中性β-甘露聚糖酶由从土壤中分离得到的枯草芽孢杆菌BM9602产生。对于该菌株，只有当多糖用作碳源时才产生中性β-甘露聚糖酶。有机氮源在酶的产生方面优于无机氮源。最佳液体培养基由4%魔芋粉、1%牛肉蛋白胨和1%酵母提取物组成。最佳培养条件为初始pH 8.5、温度35℃和培养时间36小时。在pH 6.0、50℃下作用10分钟时，培养滤液对0.5%半乳甘露聚糖多糖的酶活性为每分钟96 IU/mL。酶作用的最佳pH和温度分别为6.0和50℃。该酶在50℃以下以及pH 5.0 - 10.0范围内稳定。该酶可水解魔芋粉和刺槐豆胶形成低聚糖。
