过量活性污泥和微生物群落分析生产聚羟基丁酸酯（PHB）的优化。
Optimization of polyhydroxybutyrate (PHB) production by excess activated sludge and microbial community analysis.
机构信息
Environmental Science Research Center, College of Oceanography and Environmental Science, Xiamen University, Xiamen 361005, PR China.
出版信息
J Hazard Mater. 2011 Jan 15;185(1):8-16. doi: 10.1016/j.jhazmat.2010.08.003. Epub 2010 Aug 10.
In this study, a high value-added and biodegradable thermoplastic, polyhydroxybutyrate (PHB), was produced by excess activated sludge. The effects of the nutritional condition, aeration mode, sodium acetate concentration and initial pH value on PHB accumulation in the activated sludge were investigated. The maximum PHB content and PHB yield of 67.0% (dry cell weight) and 0.740gCODgCOD(-1) (COD: chemical oxygen demand), respectively, were attained by the sludge in the presence of 6.0gL(-1) sodium acetate, with an initial pH value of 7.0 and intermittent aeration. The analysis of the polymerase chain reaction (PCR)-denaturing gradient-gel-electrophoresis (DGGE) sequencing indicated that the microbial community of the sludge was significantly different during the process of PHB accumulation. Three PHB-accumulating microorganisms, which were affiliated with the Thauera, Dechloromonas and Competibacter lineages, were found in the excess activated sludge under different operating conditions for PHB accumulation.
在这项研究中，通过过量活性污泥生产出了一种高附加值、可生物降解的热塑性塑料——聚羟基丁酸酯（PHB）。考察了营养条件、曝气方式、乙酸钠浓度和初始 pH 值对活性污泥中 PHB 积累的影响。在 6.0 g/L 乙酸钠、初始 pH 值为 7.0 和间歇曝气的条件下，污泥的最大 PHB 含量和 PHB 得率分别达到 67.0%（干细胞重量）和 0.740 gCOD/gCOD（COD：化学需氧量）。聚合酶链反应（PCR）-变性梯度凝胶电泳（DGGE）测序分析表明，在 PHB 积累过程中，污泥的微生物群落发生了显著变化。在不同的 PHB 积累操作条件下，从过量活性污泥中发现了三种 PHB 积累微生物，它们分别属于陶厄氏菌、脱氯菌和竞争杆菌谱系。
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