Department of Microbiology, Institute of Biology, University of Bialystok, Swierkowa 20 B, 15-950 Bialystok, Poland.
Syst Appl Microbiol. 2010 Dec;33(8):457-60. doi: 10.1016/j.syapm.2010.08.007.
A total of 197 isolates of Staphylococcus from small wild animals (insectivores and rodents) were identified by partial sequencing of the rpoB and dnaJ genes. Among the identified isolates the predominant species was S. succinus (28%), followed by S. xylosus (20.8%) and S. stepanovicii (18.3%). The other 14 Staphylococcus species were occasionally isolated. PCR-RFLP of the rpoB gene digested by Hpy8I was a fast and simple method to distinguish the two subspecies of S. succinus. More than 90% of the 55 S. succinus strains isolated belonged to S. succinus subsp. casei and only 9% to S. succinus subsp. succinus. Moreover, the present study describes the first ever isolation of S. fleurettii from healthy animals.
从小型野生动物（食虫目和啮齿目动物）中共分离出 197 株葡萄球菌，通过 rpoB 和 dnaJ 基因的部分测序进行鉴定。在所鉴定的分离株中，主要的物种是 S. succinus（28%），其次是 S. xylosus（20.8%）和 S. stepanovicii（18.3%）。其他 14 种葡萄球菌偶尔分离。用 Hpy8I 消化 rpoB 基因的 PCR-RFLP 是区分 S. succinus 两个亚种的快速简单方法。从分离出的 55 株 S. succinus 菌株中，超过 90%属于 S. succinus subsp. casei，只有 9%属于 S. succinus subsp. succinus。此外，本研究首次从健康动物中分离出 S. fleurettii。
