Van Tieghem N, Vercammen-Grandjean A, Liteanu D, Lejeune F J
Ann Microbiol (Paris). 1978 Feb-Mar;129(2):217-26.
Electron microscope studies showed a high production of melanosomes and viral particles budding into the cisternae of the endoplasmic reticulum in cells derived from a subcutaneous metastasis. The history of this subline (HM6B-A) has been summarized elsewhere. An amelanotic subline (provided by the same patient) did not show similar virus particles. When infected with a vesicular stomatitis virus (VSV) thermolabile mutant (tl), these cells produced a VSV pseudotype in which a thermosensitive antigen was modified. Modification of surface VSV antigens was also detected by neutralisation tests. Using these tests, the VSV-pseudotype particles could be used as a tool to detect one or more antigens to a "putative" human melanomavirus, which might be only partially expressed.
电子显微镜研究显示，在源自皮下转移灶的细胞中，黑素小体大量产生，且有病毒颗粒出芽进入内质网池。该亚系（HM6B-A）的历史已在其他地方进行了总结。一个无黑色素亚系（由同一患者提供）未显示出类似的病毒颗粒。当用一种水疱性口炎病毒（VSV）温度敏感突变体（tl）感染这些细胞时，它们产生了一种VSV假型，其中一种温度敏感抗原发生了改变。通过中和试验也检测到了表面VSV抗原的改变。利用这些试验，VSV假型颗粒可作为一种工具，用于检测针对一种“假定的”人类黑素瘤病毒的一种或多种抗原，这种病毒可能只是部分表达。
