Zaccaria A, Celso B, Raspadori D, Motta M R, Testoni N, Rizzi S
Istituto di Ematologia L. e A. Seràgnoli, Università, Policlinico S. Orsola, Bologna, Italy.
Haematologica. 1990 Sep-Oct;75(5):412-9.
Comparative analyses of the leukocyte differential counting were performed using a Coulter VCS Hematology Flow Cytometer and direct microscopic observation on 547 unselected individuals analyzed at the outpatient clinic of the Institute of Hematology "L. e A. Seràgnoli" of Bologna. The Coulter VCS is able to provide leukocyte differential counting by measuring cell volume, conductivity and laser light scatter. Negative (true negative: 50.8%) results were observed in 278 subjects by both automated and direct observation methods. Abnormal cells in the blood samples (true positive results) were detected both by VCS and by direct observation in 199 cases (36.4%). Regional distribution of the cells in specific "flags" on the scatterplot was often associated with specific cell types. In 66 cases (12.1%) no abnormal cell was detected by direct observation, while Coulter VCS gave an abnormal pattern, even if only to a slight extent (false positive cases). In 4 cases only (0.7%), false negative results were given by the VCS system. The correlation of the results given by the VCS system with those given by direct microscopic analysis was very high; however careful control by the operator was essential in evaluating the data given by the automated system and in identifying the type of abnormal cells detected.
使用库尔特VCS血液学流式细胞仪和直接显微镜观察法，对博洛尼亚“L. e A. Seràgnoli”血液学研究所门诊分析的547名未经挑选的个体进行白细胞分类计数的比较分析。库尔特VCS能够通过测量细胞体积、电导率和激光散射来进行白细胞分类计数。通过自动和直接观察方法，在278名受试者中观察到阴性结果（真阴性：50.8%）。在199例（36.4%）中，通过VCS和直接观察均检测到血样中的异常细胞（真阳性结果）。散点图上特定“标志”中细胞的区域分布通常与特定细胞类型相关。在66例（12.1%）中，直接观察未检测到异常细胞，而库尔特VCS给出了异常模式，即使只是轻微程度（假阳性病例）。仅在4例（0.7%）中，VCS系统给出了假阴性结果。VCS系统给出的结果与直接显微镜分析给出的结果相关性非常高；然而，操作员在评估自动系统给出的数据和识别检测到的异常细胞类型时进行仔细控制至关重要。
