一种多基因转录谱分析方法用于发现细胞特征标志物。
A multi-gene transcriptional profiling approach to the discovery of cell signature markers.
机构信息
Center for Vascular Biological Research and Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 99 Brookline Avenue, Boston, MA, 02215, USA.
出版信息
Cytotechnology. 2011 Jan;63(1):25-33. doi: 10.1007/s10616-010-9315-8. Epub 2010 Oct 23.
A profile of transcript abundances from multiple genes constitutes a molecular signature if the expression pattern is unique to one cell type. Here we measure mRNA copy numbers per cell by normalizing per million copies of 18S rRNA and identify 6 genes (TIE1, KDR, CDH5, TIE2, EFNA1 and MYO5C) out of 79 genes tested as excellent molecular signature markers for endothelial cells (ECs) in vitro. The selected genes are uniformly expressed in ECs of 4 different origins but weakly or not expressed in 4 non-EC cell lines. A multi-gene transcriptional profile of these 6 genes clearly distinguishes ECs from non-ECs in vitro. We conclude that (i) a profile of mRNA copy numbers per cell from a well-chosen multi-gene panel can act as a sensitive and accurate cell type signature marker, and (ii) the method described here can be applied to in vivo cell fingerprinting and molecular diagnosis.
如果转录本丰度的表达模式是特定于某一种细胞类型的，那么这些转录本就构成了一种分子特征。在这里，我们通过标准化每百万份 18S rRNA 来测量每个细胞中的 mRNA 拷贝数，并从测试的 79 个基因中鉴定出 6 个（TIE1、KDR、CDH5、TIE2、EFNA1 和 MYO5C）作为体外内皮细胞（EC）的优秀分子特征标记基因。在 4 种不同来源的 EC 中，这些选定的基因均呈均匀表达，但在 4 种非 EC 细胞系中表达较弱或不表达。这 6 个基因的多基因转录谱可清晰地区分体外的 EC 和非 EC。我们的结论是：（i）来自精心挑选的多基因面板的每个细胞的 mRNA 拷贝数谱可以作为一种敏感且准确的细胞类型特征标记基因；（ii）这里描述的方法可应用于体内细胞指纹识别和分子诊断。
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