Department of Pathology, Medical College of Jiaying University, No. 146 Huangtang Road, Meizhou, China.
Department of Clinical Laboratory Medicine, Esophageal Cancer Prevention and Control Research Center, the Cancer Hospital of Shantou University Medical College, No. 7 Raoping Road, Shantou, China.
Sci Rep. 2024 Jul 10;14(1):15957. doi: 10.1038/s41598-024-65839-y.
We previously identified that serum EFNA1 and MMP13 were potential biomarker for early detection of esophageal squamous cell carcinoma. In this study, our aim is to explore the diagnostic value of serum EFNA1 and MMP13 for gastric cancer. We used enzyme-linked immunosorbent assay (ELISA) to detect the expression levels of serum EFNA1 and MMP13 in 210 GCs and 223 normal controls. The diagnostic value of EFNA1 and MMP13 was evaluated in an independent cohorts of GC patients and normal controls (n = 238 and 195, respectively). Receiver operating characteristics were used to calculate diagnostic accuracy. In training and validation cohorts, serum EFNA1 and MMP13 levels in the GC groups were significantly higher than those in the normal controls (P < 0.001). The area under the curve (AUC) of the combined detection of serum EFNA1 and MMP13 for GC was improved (0.794), compared with single biomarker used. Similar results were observed in the validation cohort. Importantly, the combined measurement of serum EFNA1 and MMP13 to detect early-stage GC also had acceptable diagnostic accuracy in training and validation cohort. Combined detection of serum EFNA1 and MMP13 could help identify early-stage GC, suggesting that it may be a promising tool for the early detection of GC.
我们之前发现血清 EFNA1 和 MMP13 是食管鳞状细胞癌早期检测的潜在生物标志物。在这项研究中，我们的目的是探讨血清 EFNA1 和 MMP13 对胃癌的诊断价值。我们使用酶联免疫吸附测定（ELISA）检测了 210 例 GCs 和 223 例正常对照者血清 EFNA1 和 MMP13 的表达水平。在独立的 GC 患者和正常对照组（分别为 n=238 和 195）中评估了 EFNA1 和 MMP13 的诊断价值。使用受试者工作特征曲线计算诊断准确性。在训练和验证队列中，GC 组的血清 EFNA1 和 MMP13 水平明显高于正常对照组（P<0.001）。与单独使用单一生物标志物相比，联合检测血清 EFNA1 和 MMP13 对 GC 的曲线下面积（AUC）提高（0.794）。在验证队列中也观察到了类似的结果。重要的是，联合检测血清 EFNA1 和 MMP13 对早期 GC 的诊断准确性在训练和验证队列中也可接受。联合检测血清 EFNA1 和 MMP13 有助于识别早期 GC，提示其可能是 GC 早期检测的有前途的工具。
