Adesiyun Abiodun A, Fosgate Geoffrey T, Seebaransingh Ravi, Brown Gabriel, Stoute Simone, Stewart-Johnson Alva
Faculty of Medical Sciences, School of Veterinary Medicine, University of the West Indies, St. Augustine, Trinidad and Tobago.
Trop Anim Health Prod. 2011 Jan;43(1):13-6. doi: 10.1007/s11250-010-9679-y. Epub 2010 Oct 23.
Brucellosis has been documented in domestic water buffalo (Bubalus bubalis) but published literature is limited despite the importance of this species in tropical agricultural systems. The objective of this study was to compare the virulence of Brucella abortus isolates recovered from cattle and water buffalo. Nineteen strains of B. abortus from cattle and domestic water buffalo in Trinidad were intraperitoneally inoculated into BALB/c mice. Spleens were cultured for B. abortus and histopathological severity scores were calculated based on lymphoid depletion, lymphoid necrosis, splenitis, and macrophage accumulation. A general linear model approach was used to estimate the effect of isolate source (cattle versus water buffalo) on virulence. Isolates of water buffalo origin were significantly less virulent in the mouse model based on recovered B. abortus from splenic tissues, spleen/weight ratio, and lymphoid necrosis but not overall histopathological severity scores. Further investigation of isolates recovered from water buffalo might provide the key to the development of procedures for brucellosis control in tropical environments.
布鲁氏菌病在圈养水牛（Bubalus bubalis）中已有记录，但尽管该物种在热带农业系统中很重要，已发表的文献却很有限。本研究的目的是比较从牛和水牛中分离出的布鲁氏菌流产菌株的毒力。将来自特立尼达牛和圈养水牛的19株流产布鲁氏菌经腹腔接种到BALB/c小鼠体内。对脾脏进行流产布鲁氏菌培养，并根据淋巴细胞耗竭、淋巴细胞坏死、脾炎和巨噬细胞聚集情况计算组织病理学严重程度评分。采用一般线性模型方法来估计分离株来源（牛与水牛）对毒力的影响。基于从脾脏组织中回收的流产布鲁氏菌、脾重比和淋巴细胞坏死情况，水牛来源的分离株在小鼠模型中的毒力显著较低，但总体组织病理学严重程度评分并非如此。对从水牛中分离出的菌株进行进一步研究，可能为热带环境中布鲁氏菌病控制程序的开发提供关键。
