Sinha Institute of Medical Science and Technology, 288, Kendua Main Road, Garia, Calcutta, 700 084, India.
Endocrine. 2010 Oct;38(2):294-302. doi: 10.1007/s12020-010-9388-3. Epub 2010 Oct 23.
Pancreatic β cells, stimulated by glucose, are known to synthesize and secrete insulin. As liver diseases are reported to cause diabetes mellitus, studies were conducted to determine the possibility of glucose-induced insulin synthesis in the liver cells. The glucose-induced insulin synthesis was determined by in vitro translation of mRNA from the hepatocytes. The cDNA from mRNA was prepared and sequence analysis was performed. Incubation of hepatocytes from the liver of adult mice (n=10) with glucose (0.02 M) resulted in the insulin synthesis [0.03 (mean)±0.006 (S.D.) μunits/mg/h] compared to the pancreatic β cells [0.04±0.004 μunits/mg/h]. Immunohistochemical study also demonstrated the glucose-induced synthesis of insulin in liver cells. Incubation of the mice hepatocytes with glucose resulted in the synthesis of insulin mRNA. The purified mRNA which was used to prepare cDNA resulted in the formation of proinsulin I and proinsulin II genes corresponding to 182 and 188 base pairs, respectively. Sequence analysis of the cDNA indicated that proinsulin I as well as proinsulin II gene could be involved in the synthesis of insulin by hepatocytes. These results suggested that insulin synthesis in both hepatic and pancreatic cells could be involved in the control of diabetes mellitus.
已知受葡萄糖刺激的胰腺β细胞会合成并分泌胰岛素。由于肝脏疾病会导致糖尿病，因此进行了研究以确定肝细胞内葡萄糖是否能诱导胰岛素的合成。通过对来自成年小鼠肝脏的肝细胞的 mRNA 进行体外翻译来确定葡萄糖诱导的胰岛素合成。从 mRNA 制备 cDNA 并进行序列分析。将成年小鼠肝脏的肝细胞与葡萄糖（0.02M）孵育导致胰岛素合成[0.03（平均值）±0.006（S.D.）μunits/mg/h]，与胰腺β细胞[0.04±0.004μunits/mg/h]相比。免疫组织化学研究也表明葡萄糖可诱导肝细胞合成胰岛素。将小鼠肝细胞与葡萄糖孵育可导致胰岛素 mRNA 的合成。用于制备 cDNA 的纯化 mRNA 分别产生对应于 182 和 188 个碱基对的胰岛素原 I 和胰岛素原 II 基因。对 cDNA 的序列分析表明，胰岛素原 I 和胰岛素原 II 基因都可能参与肝细胞胰岛素的合成。这些结果表明，肝和胰腺细胞中的胰岛素合成可能参与了糖尿病的控制。
