CENEXA (UNLP-CONICET LA PLATA, PAHO/WHO Collaborating Center), School of Medical Sciences, Facultad de Ciencias Médicas UNLP, Calles 60 y 120, 1900, La Plata, Argentina.
Endocrine. 2010 Oct;38(2):309-11. doi: 10.1007/s12020-010-9387-4. Epub 2010 Oct 23.
NADPH oxidase expression and activity have been measured in pancreatic islets under normal conditions, but its potential modulatory role upon insulin secretion remains unclear. We have currently studied NADPH oxidase activity in islets isolated from normal rats as well as the effect of its inhibition upon insulin secretion stimulated by different secretagogues. Glucose, arginine, fatty acids and KCl increased islet NADPH oxidase activity in a stimulus-dependent manner. DPI inhibited such increase in different proportions and affected unevenly insulin secretion, namely, it decreased the effect of high glucose, increased that of oleic acid and KCl, without changing the one induced by palmitic acid. Our data provide evidence that the contribution of NADPH activity to reactive oxygen species production in normal rat islets as well as its effect upon insulin secretion is uneven and highly stimulus-dependent.
NADPH 氧化酶的表达和活性已在正常条件下的胰岛中进行了测量，但它对胰岛素分泌的潜在调节作用仍不清楚。我们目前研究了正常大鼠胰岛中 NADPH 氧化酶的活性，以及其抑制作用对不同刺激物刺激的胰岛素分泌的影响。葡萄糖、精氨酸、脂肪酸和 KCl 以刺激依赖的方式增加胰岛 NADPH 氧化酶的活性。DPI 以不同的比例抑制这种增加，并不均匀地影响胰岛素分泌，即它降低了高葡萄糖的作用，增加了油酸和 KCl 的作用，而不改变棕榈酸诱导的作用。我们的数据提供了证据，表明 NADPH 活性对正常大鼠胰岛中活性氧产生的贡献以及对胰岛素分泌的影响是不均匀的，并且高度依赖于刺激。
