Brandys J, Lipniak M, Piekoszewski W
Akademii Medycznej im. M. Kopernika w Krakowie.
Folia Med Cracov. 1990;31(3):157-63.
In the present study we have examined the effect of treatment with benz(a)-anthracene (BaA), pyrene (P) and fluoranthene (Fl) on the total liver microsomal content of cytochrome P-450 and elimination of these PAH from blood in rats. The experiment was carried out on adult male rats Wistar breed. Oil solution of inducers: benz(a)anthracene, pyrene and fluoranthene in dose 20 mg/kg were administered individually one or threefold by gavage. 24 h after a single dose of PAH cytochrome P-450 content in rat liver determined by the method of Omura and Sato was the same as in control group. When PAH were administered p.o. for three consecutive days in the fourth day of experiment cytochrome P-450 content was increased from about 0.68 nM/mg protein to about 1.2 nM/mg. Since the increase of cytochrome P-450 may influence on elimination of xenobiotics we have determined affect PAH on their blood level after i.v. administration. One hour after BaA administration and two hours after Fl and P blood samples were collected. Concentration of PAH in blood was determined by gas-liquid chromatography. Pretreatment of rats with BaA decreased elimination of Fl, P, BaA from blood in comparison to the control rats. It indicated on the self-inducing effect of BaA on its own metabolism and inducing effect of metabolism other PAH. Pretreatment of rats with pyrene decreased elimination of BaA. It seemed that pyrene inhibited the oxidation of BaA in rat liver microsomes.
在本研究中，我们检测了用苯并(a)蒽（BaA）、芘（P）和荧蒽（Fl）处理对大鼠肝脏微粒体中细胞色素P - 450总含量以及这些多环芳烃从血液中消除的影响。实验选用成年雄性Wistar品系大鼠进行。诱导剂油溶液：苯并(a)蒽、芘和荧蒽，剂量为20 mg/kg，通过灌胃分别单次或三次给药。单次给予多环芳烃24小时后，采用大村和佐藤的方法测定大鼠肝脏中细胞色素P - 450的含量，结果与对照组相同。当在实验的第四天连续三天经口给予多环芳烃时，细胞色素P - 450的含量从约0.68 nM/mg蛋白质增加到约1.2 nM/mg。由于细胞色素P - 450的增加可能会影响外源性物质的消除，我们测定了静脉注射多环芳烃后其对血液水平的影响。给予BaA 1小时后以及给予Fl和P 2小时后采集血样。通过气液色谱法测定血液中多环芳烃的浓度。与对照大鼠相比，用BaA预处理大鼠可降低Fl、P、BaA从血液中的消除。这表明BaA对其自身代谢具有自诱导作用，对其他多环芳烃的代谢具有诱导作用。用芘预处理大鼠可降低BaA的消除。似乎芘抑制了大鼠肝脏微粒体中BaA的氧化。
