Lipniak M, Brandys J, Moniczewski A
Z Katedry Toksykologii, Akademii Medycznej im. M. Kopernika w Krakowie, Kierownik Katedry.
Folia Med Cracov. 1990;31(1-2):73-9.
We have studied in vivo and in vitro partition of pyrene, fluoranthene and benz(a)anthracene (BaA) between blood cells and plasma or phosphate buffer pH 7.4 and their binding to bovine serum albumin. The level of PAH in blood and plasma was determined in rats after i.v. administration of pyrene, fluoranthene and BaA in dose 20 mg/kg by gas chromatography method. In vitro 20 micrograms of PAH was added to blood samples or blood cell suspension. These samples were incubated at 37 degrees C. After 1 hr the concentration of PAH in blood cell and plasma or buffer was determined. The data indicate that whole pyrene and fluoranthene are distributed to blood cells. 39% of BaA in whole blood is present in blood cells and 56.9% in plasma. Plasma protein binding of PAH were determined by gel filtration 0.4 cm3 of PAH-protein solution was given on a Sephadex column. PAH binding parameters were obtained from simple linear regression of Scatchard plots of the data. In a albumin solution (0.4%) the number of binding sites was 2 for BaA, 3 for pyrene and 11 for fluoranthene. The binding constants for BaA, fluoranthene and pyrene were 0.17; 0.71 and 1.11 mol-1 X 10(4), respectively.
我们研究了芘、荧蒽和苯并(a)蒽（BaA）在血细胞与血浆或pH 7.4的磷酸盐缓冲液之间的体内外分配情况，以及它们与牛血清白蛋白的结合情况。通过气相色谱法，在大鼠静脉注射剂量为20 mg/kg的芘、荧蒽和BaA后，测定血液和血浆中多环芳烃（PAH）的水平。在体外，向血液样本或血细胞悬液中加入20微克PAH。这些样本在37℃下孵育。1小时后，测定血细胞、血浆或缓冲液中PAH的浓度。数据表明，芘和荧蒽全部分布于血细胞中。全血中39%的BaA存在于血细胞中，56.9%存在于血浆中。通过凝胶过滤法测定PAH与血浆蛋白的结合情况，将0.4 cm³的PAH - 蛋白溶液加在葡聚糖凝胶柱上。从数据的Scatchard图的简单线性回归中获得PAH结合参数。在白蛋白溶液（0.4%）中，BaA的结合位点数为2，芘为3，荧蒽为11。BaA、荧蒽和芘的结合常数分别为0.17；0.71和1.11 mol⁻¹×10⁴。
