Department of Pharmacology, Medical School, University of Athens, M. Asias 75, 11527 Athens, Greece.
Inflamm Res. 2011 Mar;60(3):265-70. doi: 10.1007/s00011-010-0264-7. Epub 2010 Oct 24.
To evaluate lymphocyte activation following mitogen and cyclosporin A (CsA) administration in peripheral blood of hyperthyroxinaemic and hypothyroid patients.
Lymphocyte activation was evaluated by determining blastogenesis in 48 h cultured blood lymphocytes obtained from eight hyperthyroxinaemic and eight hypothyroid patients, following phytohaemagglutinin (PHA)-induced stimulation in the absence or presence of CsA. Twelve healthy volunteers served as controls.
Lymphocytes from hypothyroid patients exhibited reduced response to PHA and lower sensitivity to CsA compared with control, which could be attributed to their reduced activation capability coexisting with hypothyroidism. In hyperthyroxinaemic samples, the actions of high CsA concentrations were mostly targeted toward activated lymphoblasts. Considering the cellular targets that thyroid hormones and CsA may share, the therapeutic implications of their cross-talk need careful consideration.
评估促甲状腺素原和环孢素 A（CsA）给药后甲状腺功能亢进和甲状腺功能减退患者外周血中淋巴细胞的激活情况。
通过测定植物血凝素（PHA）诱导刺激下培养 48 小时的血液淋巴细胞的增生情况，评估淋巴细胞的激活情况，来自 8 例甲状腺功能亢进和 8 例甲状腺功能减退患者，在不存在或存在 CsA 的情况下。12 名健康志愿者作为对照。
与对照相比，甲状腺功能减退患者的淋巴细胞对 PHA 的反应性降低，对 CsA 的敏感性降低，这可能归因于其在合并甲状腺功能减退时激活能力降低。在甲状腺功能亢进的样本中，高 CsA 浓度的作用主要针对激活的淋巴母细胞。考虑到甲状腺激素和 CsA 可能共享的细胞靶标，需要仔细考虑它们相互作用的治疗意义。
